海南汉族和黎族人群PCSK9基因E670G多态性与急性心肌梗死的相关性  

E670G Polymorphism of Pcsk9 Gene of Patients with Acute Myocardial Infarction among Han and Li Population in Hainan

作　　者：陈林 潘闽 刘月丽[2] 姚震 CHEN Lin;PAN Min;LIU Yueli;YAO Zhen(Department of Cardiology,Sanya People's Hospital,West China Hospital of Sichuan University,Sanya,Hainan 572000,China;Department of Pharmacology,School of Basic Medicine and Life Sciences,Hainan Medical University,Haikou 570102,China;Department of Cardiology,Geriatric Hospital of Hainan,Haikou 570102,China)

机构地区：[1]三亚市人民医院心内科四川大学华西三亚医院,海南三亚572022 [2]海南医学院药理学教研室,海口571199 [3]海南省老年病医院心脏中心,海口571199

出　　处：《岭南心血管病杂志》2024年第2期147-153,共7页South China Journal of Cardiovascular Diseases

基　　金：三亚市医疗卫生科技创新项目(项目编号:2016YW04);三亚市高校及医疗机构专项科技计划(项目编号:2021GXYL21)。

摘　　要：目的研究海南地区黎、汉族人群PCSK9基因E670G多态性与急性心肌梗死的相关性,为不同种族人群心肌梗死的预防、治疗提供科学基础依据。方法选取海南地区急性心肌梗死患者210例(黎族104例,汉族106例)为实验组、健康人群223名(黎族110名,汉族113名)为对照组,测定两组研究对象血清三酰甘油(triacylglycerol,TG)、总胆固醇(total cholesterol,TC)、低密度脂蛋白胆固醇(low-density lipoprotein-cholesterol,LDL-C)、高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)浓度,采用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction linked restriction fragment length polymorphism,PCR-RFLP)检测PCSK9基因E670G酶切位点多态性。结果心肌梗死组患者血压、空腹血糖(fasting blood glucose,FBG)、TG、TC、LDL-C浓度高于健康对照组,HDL-C浓度低于健康对照组,差异有统计学意义(P<0.05)。心肌梗死组患者PCSK9基因AG、GG基因型频率及G等位基因频率高于健康组,差异有统计学意义(P<0.05)。黎族、汉族心肌梗死组患者AG、GG基因型频率、G等位基因频率均高于健康组,差异有统计学意义(P<0.05)。黎族心肌梗死组患者GG基因型频率、G等位基因频率低于汉族心肌梗死组,差异有统计学意义(P<0.05)。黎族、汉族健康对照组GG基因型频率、G等位基因频率比较,差异无明显统计学意义(P>0.05)。心肌梗死组中GG基因型患者的TG、TC、LDL-C浓度高于AA基因型;AG与AA、GG与AG基因型患者间TG、TC、HDL-C、LDL-C浓度比较,差异无统计学意义(P>0.05),但与AA基因型相比,G等位基因携带者有较高的TG、TC、LDL-C浓度趋势。结论海南地区黎、汉族人群PCSK9基因E670G多态性与急性心肌梗死及血脂浓度密切相关;海南地区黎、汉族人群急性心肌梗死患者PCSK9基因E670G多态性存在差异。Objectives To study the correlation between the E670G polymorphism of the PCSK9 gene and acute myocardial infarction in the Li and Han populations in Hainan,providing scientific basis for the prevention and treatment of myocardial infarction in different ethnic groups.Methods A cohort of 433 participants was recruited(210 acute myocardial infarction patients and 223 healthy controls)from the Li and Han ethnic groups.The serum concentrations of triglyceride(TG),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C)and low-density lipoprotein cholesterol(LDL-C)were tested and polymerase chain reaction linked restriction fragment length polymorphism(PCR-RFLP)method was used to test the E670G polymorphism of PCSK9 gene.Results The systolic blood pressure,diastolic blood pressure,fasting blood glucose(FBG),TC,TG,and LDL-C in myocardial infarction group were higher than those in healthy control group,while the serum concentration of HDL-C in myocardial infarction group was lower than that in healthy control group,with statistical significance(P<0.05).The AG,GG genotype frequencies,and G allele frequency of the PCSK9 gene in myocardial infarction group were higher than those in healthy group(P<0.05).The frequencies of AG,GG genotypes,and G allele in the Li and Han myocardial infarction groups were higher than those in healthy group(P<0.05).The GG genotype frequency and G allele frequency in the Li ethnic group with myocardial infarction were lower than those in the Han ethnic group with myocardial infarction(P<0.05).There was no statistically significant difference in GG genotype frequency and G allele frequency between the Li and Han healthy control groups(P>0.05).The serum concentrations of TG,TC,and LDL-C in the GG genotype were higher than those in the AA genotype in myocardial infarction group(P<0.05).There was no statistically significant difference in serum concentrations of TG,TC,HDL-C,and LDL-C between AG and AA,GG and AG genotypes(P>0.05),but compared with AA genotype patients,G allele carriers show

关 键 词：心肌梗死 PCSK9基因E670G多态性 黎族 种族差异 

分 类 号：R542.22[医药卫生—心血管疾病]