基于STAT3/VEGF信号通路探究头顶一颗珠正丁醇萃取物对肝癌大鼠的抑制作用及其机制  

Exploring the Inhibitory Effect and Mechanism of N-Butanol Extract from Trillium Tschonoskii on Liver Cancer in Rats Based on the STAT3/VEGF Signaling Pathway

作　　者：彭金香 徐新华 吴广阳 姚红梅 吴沣 PENG Jin-xiang;XU Xin-hua;WU Guang-yang;YAO Hong-mei;WU Feng(Medical Department of Enshi University,Enshi Hubei 445000,China;Department of Pulmonary Disease,Enshi Tujia and Miao Autonomous Prefecture Central Hospital,Enshi Hubei 445000,China)

机构地区：[1]湖北恩施学院医学部,湖北恩施445000 [2]恩施土家族苗族自治州中心医院肺病科,湖北恩施445000

出　　处：《中华养生保健》2024年第12期9-13,共5页CHINESE HEALTH CARE

基　　金：2022年恩施州科技计划项目(JCY2022000043);2022年度教育部产学合作协同育人项目(220505181164558);2023年度湖北恩施学院教师类项目(KYJZ202302)。

摘　　要：目的探讨头顶一颗珠正丁醇萃取物对二乙基亚硝胺(DEN)诱发的大鼠原发性肝癌的抑制作用,探讨其对STAT3/VEGF信号通路的影响,并研究其对抗肝癌血管生成的分子机制。方法将大鼠随机分为G1组(正常对照组)、G2组(模型组)、G3组(华蟾素组)、G4组(头顶一颗珠低剂量组)、G5组(头顶一颗珠高剂量组),除G1组外所有大鼠腹腔注射二乙基亚硝胺建立肝癌大鼠模型。使用头顶一颗珠正丁醇萃取物处理肝癌大鼠,检测大鼠肝脏指数、脾脏指数;检测大鼠的血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、白蛋白(ALB)和总胆红素(TBIL)的水平;ELISA法检测血清TGF-β1和VEGF的水平;同时采用Western blot检测VEGF、KDR、STAT3、p-STAT3蛋白水平。结果与G1组比较,G2组大鼠肝脏指数、ALT、AST和TBIL的水平显著增加,ALB的水平显著降低,血清TGF-β1和VEGF的水平显著升高,肝组织VEGF、KDR、STAT3、p-STAT3蛋白水平显著升高,差异有统计学意义(P<0.05);与模型组比较,头顶一颗珠正丁醇萃取物组大鼠肝脏指数显著降低,血清AST和TBIL水平显著降低,血清TGF-β1和VEGF的水平显著降低,肝组织VEGF、KDR、STAT3、p-STAT3蛋白水平显著降低,差异有统计学意义(P<0.05)。结论头顶一颗珠正丁醇萃取物通过抑制STAT3/VEGF信号通路,发挥抗肝癌血管生成作用。Objective To investigate the inhibitory effect of Trillium tschonoskii Maxim N-butanol extract on diethylnitrosamine(DEN)-induced primary liver cancer in rats,explore its impact on the STAT3/VEGF signaling pathway,and study its molecular mechanisms against liver cancer angiogenesis.Methods Rats were randomly divided intofive groups:G1(normal control group),G2(model group),G3(silibinin group),G4(Trillium tschonoskii Maxim low-dose group),and G5(Trillium tschonoskii Maxim high-dose group).Except for the G1 group,all rats were intraperitoneally injected with DEN to establish a liver cancer rat model.Trillium tschonoskii Maxim N-butanol extract was used to treat liver cancer rats,and the liver index and spleen index of rats were measured.The levels of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),albumin(ALB),and total bilirubin(TBIL)were determined.ELISA was used to detect the levels of serum transforming growth factor-beta 1(TGF-β1)and vascular endothelial growth factor(VEGF).Western blot was used to detect the levels of VEGF,kinase insert domain receptor(KDR),STAT3,and phosphorylated STAT3(p-STAT3)proteins.Results Compared with the G1 group,the levels of liver index,ALT,AST,and TBIL were significantly increased in the G2 group,while the level of ALB was significantly decreased.The levels of serum TGF-β1 and VEGF were significantly increased,and the protein levels of VEGF,KDR,STAT3,and p-STAT3 in liver tissues were significantly increased(P<0.05).Compared with the model group,the liver index of rats in the Trillium tschonoskii Maxim N-butanol extract group was significantly decreased,and the levels of serum AST and TBIL were significantly decreased.The levels of serum TGF-β1 and VEGF were significantly decreased,and the protein levels of VEGF,KDR,STAT3,and p-STAT3 in liver tissues were significantly decreased(P<0.05).Conclusion Trillium tschonoskii Maxim N-butanol extract exerts anti-liver cancer angiogenesis effects by inhibiting the STAT3/VEGF signaling pathway.

关 键 词：头顶一颗珠正丁醇萃取物 肝癌 STAT3 血管生成 VEGF KDR 

分 类 号：R285.5[医药卫生—中药学]