红景天苷通过Wnt/β-catenin信号通路对肺腺癌A549细胞的影响  

Effects of Salidroside on Lung Adenocarcinoma A549 Cells via Wnt/β-catenin Signaling Pathway

作　　者：郭贺华 曾玉兰 聂雅兰 周薇 GUO He-hua;ZENG Yu-lan;NIE Ya-lan;ZHOU Wei(Department of Respiratory Medicine,Liyuan Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan Hubei 430077)

机构地区：[1]华中科技大学同济医学院附属梨园医院呼吸内科,湖北武汉430077

出　　处：《世界中西医结合杂志》2024年第5期927-932,共6页World Journal of Integrated Traditional and Western Medicine

摘　　要：目的 探讨红景天苷对肺腺癌A549细胞的增殖、迁移、细胞周期及凋亡的影响及其作用机制。方法 将红景天苷作用于人肺腺癌A549细胞,采用细胞划痕实验及Transwell实验检测其对细胞的增殖迁移作用。采用Hoechst 33342荧光染色实验及Annexin V-FITC/PI双染染色实验检测细胞凋亡。采用碘化丙啶染色检测细胞周期。采用Western blot实验检测Bax、Bcl2、P21、CyclinD1、Wnt3a、β-catenin蛋白的表达。采用实时荧光定量PCR实验检测Bax、Bcl2、P21、CyclinD1、Wnt3a、β-catenin基因的表达。结果 红景天苷可以抑制A549细胞的增殖迁移,抑制细胞周期阻滞在G1/G2期,同时诱导细胞凋亡,促进促凋亡蛋白和基因的表达,抑制抗凋亡蛋白和基因的表达,抑制与转录有关的Wnt3a、β-catenin蛋白基因的表达。结论 红景天苷对肺腺癌A549细胞具有抑制细胞增殖、迁移、细胞周期,诱导细胞凋亡的作用,可能与其下调Wnt/β-catenin信号通路有关。Objective This study aims to investigate the effects of salidroside on the proliferation,migration,cell cycle,and apoptosis of lung adenocarcinoma A549 cells,as well as the mechanism of action of salidroside.Methods The human lung adenocarcinoma A549 cells were treated with salidroside,and the effect of salidroside on cell proliferation and migration was detected by cell scratch assay and Transwell assay.Cell apoptosis was detected by Hoechst 33342 fluorescent staining assay and Annexin V-FITC/PI double staining assay.The cell cycle was detected by propidium iodide staining.The protein expressions of Bax,Bcl2,P21,CyclinD1,Wnt3a,and β-catenin were detected by Western blot.The gene expression of Bax,Bcl2,P21,CyclinD1,Wnt3a,and β-catenin was detected by real-time quantitative polymerase chain reaction(PCR).Results Salidroside could inhibit the proliferation and migration of A549 cells,make cell cycle arrest in the G1/G2 phase,induce apoptosis,promote the expression of pro-apoptotic proteins and genes,and inhibit the expression of anti-apoptotic proteins and genes and the expression of Wnt3a and β-catenin proteins and genes related to transcription.Conclusion Salidroside could inhibit proliferation,migration,and cell cycle and induce apoptosis in lung adenocarcinoma A549 cells,which might be related to the down-regulation of the Wnt/β-catenin signaling pathway.

关 键 词：红景天苷 肺腺癌 WNT Β-CATENIN 

分 类 号：R285.5[医药卫生—中药学]