临床生化检验标本溶血对肝肾功能、血脂和心肌标志物的影响及应对措施  

Effects of hemolysis of clinical biochemical test specimens on liver and kidney function,blood lipid and myocardial markers and countermeasures

作　　者：崔亚利[1] 杨延敏[1] 陈永传 赵雪银 CUI Yali;YANG Yanmin;CHEN Yongchuan;ZHAO Xueyin(Laboratory Department,Beijing Fengtai Hospital,Beijing 100071;Laboratory Department,Beijing Sanfine International Hospital,Beijing 100027;Cardiology Department,Beijing Fengtai Hospital,Beijing 100071,China)

机构地区：[1]北京丰台医院检验科,北京100071 [2]北京善方医院检验科,北京100027 [3]北京丰台医院心内科,北京100071

出　　处：《临床医学研究与实践》2024年第17期117-120,共4页Clinical Research and Practice

摘　　要：目的探讨临床生化检验标本溶血对肝肾功能、血脂和心肌标志物的影响及应对措施。方法选择2021年3月至2022年9月接受生化检验的120例健康体检者,在晨起空腹状态下采集肘部静脉血10 mL作为检测标本,将每位体检者的血液标本均分为2份,分别设置为观察组与对照组。观察组血液样本经溶血处理后行临床生化检验,对照组血液样本未经溶血处理,直接行临床生化检验。比较两组健康体检者的肝功能、心肌标志物、肾功能及血脂指标。结果观察组的天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)及总蛋白(TP)水平明显高于对照组,差异具有统计学意义(P<0.05)。观察组的肌酸激酶同工酶MB(CK-MB)、肌酸激酶(CK)及N末端脑钠肽前体(NT-proBNP)水平明显高于对照组,差异具有统计学意义(P<0.05)。观察组的血尿素氮(BUN)、血清肌酐(Scr)及尿酸(UA)水平明显高于对照组,差异具有统计学意义(P<0.05)。观察组的甘油三酯(TG)、总胆固醇(TC)水平高于对照组,差异具有统计学意义(P<0.05);两组的高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平比较,差异无统计学意义(P>0.05)。结论临床生化检验血液标本发生溶血反应会影响肝肾功能、血脂、心肌标志物等检验项目的检测结果,易导致生化检验结果误差,临床诊疗工作中对此需充分重视,并制定一系列应对措施。Objective To investigate the effects of hemolysis of clinical biochemical test specimens on liver and kidney function,blood lipids and myocardial markers and countermeasures.Methods A total of 120 healthy objects who underwent biochemical tests from March 2021 to September 2022 were selected.10 mL of elbow venous blood was collected as a test sample in the morning on an empty stomach.The blood samples of each physical examinee were divided into 2 samples,which were set as observation group and control group.The blood samples of the observation group were subjected to clinical biochemical tests after hemolysis treatment,and the blood samples of the control group were directly subjected to clinical biochemical tests without hemolysis treatment.The liver function,myocardial markers,renal function and blood lipid indexes of healthy objects in the two groups were compared.Results The levels of aspartate aminotransferase(AST),alanine aminotransferase(ALT)and total protein(TP)in the observation group were significantly higher than those in the control group,and the differences were statistically significant(P<0.05).The levels of creatine kinase MB isoenzyme(CK-MB),creatine kinase(CK)and N-terminal pro-brain natriuretic peptide(NT-proBNP)in the observation group were significantly higher than those in the control group,and the differences were statistically significant(P<0.05).The levels of blood urea nitrogen(BUN),serum creatinine(Scr)and uric acid(UA)in the observation group were significantly higher than those in the control group,and the differences were statistically significant(P<0.05).The levels of triglyceride(TG)and total cholesterol(TC)in the observation group were higher than those in the control group,and the differences were statistically significant(P<0.05);there were no significant differences in the levels of high-density lipoprotein cholesterol(HDL-C)and low-density lipoprotein cholesterol(LDL-C)between the two groups(P>0.05).Conclusion Hemolytic reaction of blood samples in clinical biochemical test wil

关 键 词：肝肾功能 生化检验 血脂 标本 溶血 应对措施 心肌标志物 

分 类 号：R446.1[医药卫生—诊断学]