益母草碱对ox-LDL诱导的THP-1源性巨噬细胞焦亡的保护作用  

Protective effects of epsilonine on ox-LDL-induced pyroptosis of THP-1-derived macrophages

作　　者：杨依壮 陈旭[1] 艾锐 侯新月 王娟[1] YANG Yizhuang;CHEN Xu;AI Rui;HOU Xinyue;WANG Juan(College of Pharmacy,Guilin Medical University,Guilin 541199,China)

机构地区：[1]桂林医学院药学院,桂林541199

出　　处：《华夏医学》2024年第2期46-53,共8页Acta Medicinae Sinica

基　　金：广西科技基地和人才专项(桂科AD20238024);广西科技厅中央引导地方科技发展资金项目(桂科ZY21195024);广西科技计划基金项目(桂科ZD20302006);第四批八桂学者2017年专项(〔2017〕143号)。

摘　　要：目的探讨益母草碱(LEO)对氧化低密度脂蛋白(ox-LDL)诱导的THP-1源性巨噬细胞焦亡的保护作用。方法采用MTT法检测不同浓度LEO对THP-1巨噬细胞/THP-1源性泡沫细胞活力;采用油红O染色评估细胞内脂质蓄积的情况,并检测细胞内总胆固醇(TC)、甘油三酯(TG)的含量以及细胞上清液中白细胞介素-1β(IL-1β)、白细胞介素-18(IL-18)和乳酸脱氢酶(LDH)含量;采用Western blot检测细胞焦亡相关蛋白的表达水平;采用流式细胞术测定活性氧(ROS)水平。结果随着LEO浓度的增加,细胞活性与LEO的浓度呈正相关。LEO干预后,细胞内TC和TG的含量降低,细胞内脂质积累减少,且LEO可下调细胞焦亡相关蛋白NLRP3、N-GSDMD、Cleaved Caspase-1的表达,同时经LEO处理可逆转ox-LDL诱导的ROS生成增加。结论LEO可以减缓由ox-LDL诱导的THP-1巨噬细胞内脂质积累,并且可以抑制ox-LDL诱导的THP-1巨噬细胞焦亡的发生。Objective To investigate the protective effects of leonurine(LEO)on pyroptosis induced by oxidized lowdensity lipoprotein(ox-LDL)in THP-1-derived macrophages.Methods MTT assay was used to detect the viability of THP-1 macrophages/THP-1-derived foam cells after treatment with different concentrations of LEO.Oil Red O staining was used to assess the intracellular lipid accumulation,meanwhile the contents of intracellular total cholesterol(TC),triglycerides(TG),interleukin-1β(IL-1β),interleukin-18(IL-18),and lactate dehydrogenase(LDH)in the cell supernatan were detected.Western blot was used to detect the expression level of proteins related to cellular pyrolysis.The level of reactive oxygen species(ROS)was determined by flow cytometry.Results As the concentration of LEO increased,there was a positive correlation between cell activity and LEO concentration.LEO intervention decreased the intracellular TC and TG levels,and reduced the intracellular lipid accumulation.LEO decreased the expression of cellular pyroptosis-related proteins,including NLRP3,N-GSDMD,Cleaved Caspase-1.At the same time,treatment with LEO reversed the ox-LDL-induced increase in ROS production.Conclusion LEO decreases lipid accumulation in THP-1 macrophages induced by ox-LDL and inhibits the pyroptosis induced by ox-LDL in THP-1-derired macrophage.

关 键 词：益母草碱 巨噬细胞 氧化低密度脂蛋白 细胞焦亡 

分 类 号：R285.5[医药卫生—中药学]