基于转录组学探讨水蛭素对HK-2细胞的影响机制  

Exploring the influencing mechanisms of hirudin on HK-2 cells based on transcriptomics

作　　者：何华梅 赵应学[2] 吴林秀[1] 周维海 刘喜华[1] 甄汉深[4] HE Huamei;ZHAO Yingxue;WU Linxiu;ZHOU Weihai;LIU Xihua;ZHEN Hanshen(Guangxi Health Science College,Nanning 530023,China;Jiangbin Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,China;Guangxi Kekang Technology Group Co.,Ltd,Nanning 530001,China;Guangxi University of Traditional Chinese Medicine,Nanning 530001,China)

机构地区：[1]广西卫生职业技术学院,南宁530023 [2]广西壮族自治区江滨医院,南宁530021 [3]广西科康科技集团有限公司,南宁530001 [4]广西中医药大学,南宁530001

出　　处：《华夏医学》2024年第2期87-94,共8页Acta Medicinae Sinica

基　　金：广西自然科学基金项目(2018GXNSFAA281093);广西高校中青年教师科研基础能力提升项目(2020KY43012)。

摘　　要：目的采用转录组学技术探讨水蛭素对人肾皮质近曲小管上皮细胞(HK-2细胞)的影响及机制。方法利用CCK-8法检测水蛭素处理之后的HK-2细胞活力,通过转录组学的技术对溶媒对照组和水蛭素组进行转录组测序;通过RSEM的方法计算不同样品中的基因表达水平,差异基因的筛选采用DEseq2方法,筛选标准为|log2FC值|≥1,P<0.05。随后根据差异表达的基因进行富集分析。结果水蛭素5 mg/mL下提高HK-2细胞活力效果最佳,转录组学研究结果表明,与正常HK-2细胞相比,水蛭素处理后的HK-2细胞有1723个基因发生差异表达。GO和KEGG分析结果表明,炎症通路的调控是水蛭素治疗高尿酸血症的主要作用机制。结论水蛭素作用于多条炎症信号通路,进而促进尿酸排泄,促进集体代谢和尿酸排出体外,起到降尿酸的作用,表明水蛭素在治疗高尿酸血症方面效果显著。Objective To explore the effects and mechanisms of hirudin on human kidney-2 cell(HK-2 cells)using transcriptomics techniques.Methods The CCK-8 method was used to detect the viability of HK-2 cells,and transcriptome sequencing was performed in the solvent control group and hirudin group.The gene expression levels in different samples were calculated using the RSEM method,and differential gene screening was performed using the DEseq2 method with a screening criterion of|log2FC|≥1 and P<0.05.Subsequently,enrichment analysis was performed based on differentially expressed genes.Results The hirudin(5 mg/mL)had the best effect on enhancing the viability of HK-2 cells.Transcriptomic studies found that compared with normal HK-2 cells,there were 1723 genes were differentially expressed in HK-2 cells treated with hirudin.GO and KEGG analysis results showed that the regulation of inflammatory pathways was the main mechanism by which hirudin increased HK-2 cels viability.Conclusion Hirudin increases the viability of HK-2 cells,and its mechanism may be involved the regulation of inflammatory pathways.

关 键 词：水蛭素 人肾皮质近曲小管上皮细胞 转录组 

分 类 号：R285.5[医药卫生—中药学]