PBK/TOPK在类风湿关节炎患者单核细胞中的表达及临床意义  

Expression and clinical significance of PBK/TOPK in monocytes of patients with rheumatoid arthritis

作　　者：王琪 覃泱[3] 臧念可 刘瑜 辛林伟 魏兵 WANG Qi;QIN Yang;ZANG Nianke;LIU Yu;XIN Linwei;WEI Bing(College of Public Health,Guilin Medical University,Guilin 541100,China;Guangxi Key Laboratory of Tumor Immune and Microenvironmental Regulation,Guilin 541199,China;Affiliated Hospital of Guilin Medical University,Guilin 541001,China;The Second Affiliated Hospital of Guilin Medical University,Guilin 541199,China)

机构地区：[1]桂林医学院公共卫生学院,桂林541199 [2]广西肿瘤免疫与微环境调控重点实验室,桂林541199 [3]桂林医学院附属医院,桂林541001 [4]桂林医学院第二附属医院,桂林541199

出　　处：《华夏医学》2024年第2期95-102,共8页Acta Medicinae Sinica

基　　金：国家自然科学基金地区科学基金项目(82360330);广西自然科学基金面上项目(2020JJA140128);广西脑与认知神经科学重点实验室2020年度开放课题(GKLBCN-20200108-06);广西肿瘤免疫与微环境调控重点实验室开放课题(2202KF004);广西医疗卫生重点培育学科建设项目。

摘　　要：目的研究PDZ结合激酶/T-淋巴因子激活的杀伤细胞来源的蛋白激酶(PBK/TOPK)在类风湿关节炎(RA)患者单核细胞的表达及临床意义。方法以47例RA患者、20例健康体检者为研究对象,采用流式细胞术检测外周血单个核细胞(PBMC)中PBK/TOPK的表达。结果PBK/TOPK在RA患者CD14^(+)单核细胞表达水平高于健康体检者,差异具有统计学意义(P<0.05)。RA患者CD14^(+)单核细胞PBK/TOPK低表达组CD14^(+)单核细胞在PBMC中所占比例、白细胞(WBC)计数、类风湿因子(RF)、免疫球蛋白A(IgA)、免疫球蛋白G(IgG)的水平均高于PBK/TOPK高表达组,差异具有统计学意义(P<0.05)。RA住院患者单核细胞PBK/TOPK表达低于门诊患者,且住院患者RF水平高于门诊患者,差异具有统计学意义(P<0.05)。结论单核细胞PBK/TOPK低表达与疾病炎症有关,对类风湿关节炎的靶向治疗有一定意义。Objective To investigate the expression and clinical significance of PDZ-binding-kinase/T-LAK cellderived protein kinase(PBK/TOPK)in monocytes of patients with rheumatoid arthritis(RA).Methods PBK/TOPK expression in peripheral blood mononuclear cells(PBMC)was detected by flow cytometry in 47 RA patients and 20 healthy subjects.Results The expression level of PBK/TOPK in peripheral blood CD14^(+)monocytes in RA patients was higher than that in healthy subjects,and the difference was statistically significant(P<0.05).The proportion of CD14^(+)monocytes in PBMC,white blood cell(WBC)count,rheumatoid factor(RF),immunoglobulin A(IgA)and immunoglobulin G(IgG)of CD14^(+)monocytes in RA patients with low expression of PBK/TOPK were higher than those in RA patients with high expression of PBK/TOPK.The difference was statistically significant(P<0.05).The monocyte PBK/TOPK expression in inpatients with RA was lower than that in outpatients,and the RF level in inpatients was higher than that in outpatients.The difference was statistically significant(P<0.05).Conclusion The low expression of PBK/TOPK in monocytes is related to the inflammation of the disease,and it has certain significance for the targeted therapy of RA.

关 键 词：PBK/TOPK 类风湿关节炎 单核细胞 

分 类 号：R593.22[医药卫生—内科学]