NFAT3在充血性心力衰竭气虚血瘀证患者舌苔液中的表达及意义  

Expression and Significance of NFAT3 in Tongue Coating Fluid of Congestive Heart Failure with Qi Deficiency and Blood Stasis Syndrome

作　　者：张蕾[1] 李小茜[2] 何建成[2] ZHANG Lei;LI Xiaoqian;HE Jiancheng(The First Affiliated Hospital,College of Clinical Medicine of Henan University of Science and Technology,Luoyang 471003,Henan,China;Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]河南科技大学临床医学院,河南科技大学第一附属医院,河南洛阳471003 [2]上海中医药大学,上海201203

出　　处：《辽宁中医杂志》2024年第6期10-13,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金青年项目(81803996);河南省科技研发计划联合基金项目(232103810046);河南省医学科技攻关计划联合共建项目(LHGJ20230468)。

摘　　要：目的研究活化T细胞核因子3(nuclear factor of activated T-cells 3,NFAT3)在充血性心力衰竭(congestive heart failure,CHF)气虚血瘀证患者舌苔液中的表达及与临床指标相关性,初步拟定该证候的诊断界值。方法收集CHF气虚血瘀证患者及健康对照者各30例,酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)检测舌苔液中NFAT3的含量。结果NFAT3在CHF气虚血瘀证患者舌苔液的含量为(481.40±103.00)pg/mL,明显高于健康对照者舌苔液的含量[(237.90±156.50)pg/mL](P<0.01);且随着美国纽约心脏协会(New York Heart Association,NYHA)心功能分级的增加,CHF气虚血瘀证患者舌苔液中NFAT3含量不断升高(P<0.01);CHF气虚血瘀证患者舌苔液NFAT3与NYHA心功能分级呈正相关性(r=0.927,P<0.01),与B型脑钠肽(brain natriuretic peptide,BNP)(r=0.806,P<0.01)呈正相关性,与左室射血分数(left ventricular ejection fraction,LVEF)呈负相关性(r=-0.739,P<0.01)。绘制受试者操作特性曲线(receive operating characteristic,ROC)初步拟定舌苔液清中NFAT3诊断CHF气滞血瘀证的界值为363.37 pg/mL,曲线下面积为0.91。结论NFAT3能够反映CHF病情轻重程度,可为CHF气虚血瘀证提供客观化、量化的参考和依据。Objective To study the expression of nuclear factor of activated T-cells 3(NFAT3)in tongue coating fluid of patients with congestive heart failure(CHF)of Qi deficiency and blood stasis syndrome and its correlation with clinical indicators and preliminarily draw up the diagnostic threshold of this syndrome.Methods A total of 30 patients with CHF of Qi deficiency and blood stasis syndrome and 30 healthy controls were collected.The contents of NFAT3 in tongue coating fluid were determined by ELISA.Results The content of NFAT3 in tongue coating fluid of CHF patients with Qi deficiency and blood stasis syndrome was(481.40±103.00)pg/mL,significantly higher than that of the healthy control group[(237.90±156.50)pg/mL](P<0.01).Moreover,the content of NFAT3 increased with the increase of cardiac function classification(P<0.01).In patients with CHF of Qi deficiency and blood stasis syndrome,there was a positive correlation between NFAT3 and cardiac function classification(r=0.927,P<0.01).It was positively correlated with brain natriuretic peptide(BNP)(r=0.806,P<0.01)and negatively correlated with left ventricular ejection fraction(LVEF)(r=-0.739,P<0.01).The receiver operating characteristic(ROC)was drawn to preliminarily determine the diagnostic threshold value of NFAT3 in tongue coating fluid for diagnosing CHF of Qi stagnation and blood stasis syndrome,which was 363.37 pg/mL and the AUC was 0.91.Conclusion NFAT3 can reflect the severity of CHF and provide an objective and quantitative reference for Qi deficiency and blood stasis syndrome of CHF.

关 键 词：充血性心力衰竭 气虚血瘀证 舌苔液 活化T细胞核因子3 诊断界值 

分 类 号：R256.2[医药卫生—中医内科学]