地龙蛋白对糖尿病性勃起功能障碍大鼠阴茎海绵体平滑肌细胞表型转化的影响  

Effect of lumbricus protein on the phenotypic transformation of penile corpus cavernosum smooth muscle cells in diabetic mellitus erectile dysfunction rats

作　　者：冀小卫 辛学燕 舒译 张爱平 刘黎明 杨佳树 邢喜平 JI Xiaowei;XIN Xueyan;SHU Yi;ZHANG Aiping;LIU Liming;YANG Jiashu;XING Xiping(Master Degree Candidate of Integrative Medicine Enrolled in 2021,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,Gansu,China;Department of Reproduction,Hongji Hospital of Traditional Chinese Medicine,Zouping City,Binzhou 265200,Shandong,China;Master Degree Candidate of Chinese Medicine Surgery Enrolled in 2019,Changsha 410000,Hunan,China;Department of Andrology,Affiliated Hospital of Gansu University of Traditional Chinese Medicine,Lanzhou 730000,Gansu,China)

机构地区：[1]甘肃中医药大学,兰州730000 [2]邹平市洪济中医医院生殖科,山东滨州265200 [3]湖南中医药大学,长沙410000 [4]甘肃中医药大学附属医院男科,兰州730000

出　　处：《中国性科学》2024年第5期1-6,共6页Chinese Journal of Human Sexuality

基　　金：2023年兰州市科技发展指导性计划项目(2023-ZD-102);第一批陇原青年英才培养项目(甘人社通〔2021〕404号);甘肃省卫生健康行业骨干人才项目(甘卫人函〔2023〕398号)。

摘　　要：目的探讨地龙蛋白对糖尿病性勃起功能障碍(DMED)大鼠阴茎海绵体平滑肌细胞(CCSMC)表型转化的影响,以明确地龙蛋白改善DMED大鼠勃起功能的机制。方法于2021年7月选取60只SPF级雄性SD大鼠作为研究对象,随机选取50只腹腔注射链脲佐菌素(STZ),构建糖尿病(DM)大鼠模型,余下10只作为正常组。8周后大鼠颈部皮下注射阿朴吗啡(APO),并筛选出DMED大鼠。将DMED大鼠随机分为模型组、西地那非组及地龙蛋白低、中、高剂量组(45、90、180 mg/kg),各6只,并随机选取正常组6只作为空白组。给药4周后,检测大鼠血糖、体重、阴茎海绵体内压(ICP)和颈动脉内压(MAP)水平,计算ICP/MAP评估大鼠勃起功能。采用免疫组织化学法检测大鼠阴茎海绵体中收缩型标志物碱性调宁蛋白(Caiponin)、肌间线蛋白(Desmin)表达水平;采用实时荧光定量聚合酶链反应检测阴茎海绵体中Desmin、骨桥蛋白(OPN)mRNA表达水平;采用Western blotting法检测阴茎海绵体中Caiponin、平滑肌肌球蛋白重链(SMMHC)蛋白表达水平。结果与空白组比较,模型组大鼠体重、ICP/MAP显著下降,血糖显著升高(P<0.01);各给药组大鼠阴茎海绵体中Caiponin、Desmin表达显著下降(P<0.01);Desmin mRNA表达下降,OPN mRNA表达显著上升(P<0.01);Caiponin、SMMHC蛋白表达显著下降(P<0.01)。与模型组比较,西地那非组及地龙蛋白低、中、高剂量组大鼠ICP/MAP水平显著升高(P<0.01),但西地那非组及地龙蛋白低、中、高剂量组大鼠的体重及血糖水平比较,差异无统计学意义(P>0.05);各给药组大鼠阴茎海绵体中Caiponin、Desmin表达显著上升(P<0.01);Desmin mRNA表达显著上升,OPN mRNA表达显著下降(P<0.01);Caiponin、SMMHC蛋白表达显著上升(P<0.01)。结论地龙蛋白可通过上调阴茎海绵体组织中合成型(增殖型)标志蛋白或下调收缩型标志蛋白影响CCSMC转化,进而改善DMED大鼠的勃起功能。Objective To investigate the effect of lumbricus protein on the phenotypic transformation of penile corpus cavernosum smooth muscle cells(CCSMC)in diabetic mellitus erectile dysfunction(DMED)rats,in order to clarify the mechanism of lumbricus protein improving the erectile function in DMED rats.Methods A total of 60 specific pathogen free(SPF)-grade male sprague-dawley(SD)rats were selected as study subjects in July 2021,and 50 were randomly selected to be injected intraperitoneally with streptozotocin(STZ)to construct a diabetic mellitus(DM)rat model,and the remaining 10 were used as the normal group.Rats were injected subcutaneously with apomorphine(APO)in the neck after 8 weeks and screened for DMED rats.The DMED rats were randomly divided into the model group,sildenafil group,and the low,medium and high dose groups of lumbricus protein(45,90,and 180 mg/kg),with 6 rats in each group,and 6 rats from the normal group were randomly selected as the blank group.After 4 weeks of administration,blood glucose,body weight,penile intracavernous pressure(ICP)and carotid mean arterial pressure(MAP)levels were detected and ICP/MAP was calculated to assess the erectile function of rats.Immunohistochemistry was used to detect the expression levels of contractile markers Caiponin and Desmin in rat penile corpus cavernosum.Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the mRNA expression levels of Desmin and osteopontin(OPN)in penile corpus cavernosum.Western blotting was used to detect the protein expression levels of caiponin and smooth muscle myosin heavy chain(SMMHC)in penile corpus cavernosum.Results Compared with the blank group,the body weight and ICP/MAP of rats in the model group decreased significantly,and blood glucose increased significantly(P<0.01);the expressions of Caiponin and Desmin in the penile corpus cavernosum of the rats in each administration group decreased significantly(P<0.01);the expression of Desmin mRNA decreased,and the expression of OPN mRNA increased significantly(P

关 键 词：地龙蛋白 勃起功能障碍 糖尿病 阴茎海绵体平滑肌细胞 表型转化 

分 类 号：R698[医药卫生—泌尿科学]