木霉菌和复合微生物制剂在海三棱藨草湿地修复中的应用研究  

Studies on the applications of Trichoderma and compound microbial agents in the restoration of Scirpus mariqueter wetland

作　　者：王沁怡 张生乐 张伯伦 周志杰 何培民 方淑波 WANG Qinyi;ZHANG Shengle;ZHANG Bolun;ZHOU Zhijie;HE Peimin;FANG Shubo(College of Marine Ecology and Environment,Shanghai Ocean University,Shanghai 201306,China;College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学海洋生态与环境学院,上海201306 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《海洋湖沼通报》2024年第2期116-124,共9页Transactions of Oceanology and Limnology

基　　金：国家重大研发计划课题(2017YFC0506002);自然资源部海洋生态监测与修复技术重点实验室开放基金(202003)。

摘　　要：为探究微生物制剂促进海三棱藨草湿地修复的可行性,本研究通过南汇东滩海三棱藨草四种浓度的木霉菌、复合微生物制剂对比添加实验,分析比较了添加不同浓度的微生物制剂后海三棱藨草数量和株高增长率、根际土壤理化性状及酶活性等指标的差异,实验的A、B两种典型土壤来自南汇东滩。结果显示,两种微生物制剂在一定程度上促进了海三棱藨草的生长,在A、B两种不同土壤中,其最佳添加浓度分别为木霉菌44.78,29.85 kg/ha,复合微生物制剂7.46,14.93 L/ha,对应的植株增长率分别为669%,200%,846%,288%,株高增长率达186%,180%,136%,280%。微生物制剂通过影响土壤中的铵态氮、硝态氮、总磷含量以及土壤脲酶、碱性磷酸酶活性,促进植株的生长。研究结果表明,添加适宜的微生物制剂,可以促进海三棱藨草植株数量和株高的增长,为海三棱藨草湿地的人工修复提供了快速有效的可行性方案。In this study,the feasibility of promoting wetland restoration using the Trichoderma and the microbial agents was investigated.The analysis focused on the effects of different concentrations of Trichoderma and composite microbial agents on the characteristics of Scirpus mariqueter inhabiting Dongtan,Nanhui,which include plant number,plant height growth rate,rhizosphere soil physical and chemical properties,and enzyme activities.Two types of soils,A and B,representing the typical soil conditions were collected.The results indicated that the microbial preparations from two soil samples had a positive impact on the growth of S.mariqueter.The optimal density of Trichoderma in soils A and B was 44.78 kg/ha and 29.85 kg/ha,respectively.For the compound microbial preparations,the optimal concentrations were found to be 7.46 L/ha and 14.93 L/ha,respectively.The corresponding plant growth rate was observed to be 669%,200%,846%and 288%while the plant height growth rate was 186%,180%,136%and 280%,respectively.The addition of microbial preparations influenced the content of ammonium nitrogen,nitrate nitrogen and total phosphorus in the soil.The influenced in-cluded also the activities of soil urease and alkaline phosphatase.These changes contributed to the pro-motion of plant growth.Overall,our findings demonstrated that the application of suitable microbial a-gents can effectively enhance the number and height of S.mariqueter.What we found provided a prac-tical and efficient approach to the artificial restoration of S.mariqueter wetlands,proved the potential of wetland conservation and restoration effort.

关 键 词：滨海湿地 微生物制剂 木霉菌 土壤酶 海三棱藨草 

分 类 号：X37[环境科学与工程—环境工程]