海水铜离子污染对喜盐草生长及抗氧化酶活性的影响  

Effect of bivalent copper ion on the growth and antioxidant enzyme activity of Halophila ovalis

作　　者：李倩茹[1] 曾思琴 胡杰飞 黄俊 张玉蕾 曾奇韬 罗晓霞[1] 黄翔鹄[1] LI Qianru;ZENG Siqin;HU Jiefei;HUANG Jun;Zhang Yulei;ZENG Qitao;LUO Xiaoxia;HUANG Xianghu(School of Fisheries,Guangdong Ocean University,Zhanjiang 524000,China)

机构地区：[1]广东海洋大学水产学院,广东湛江524000

出　　处：《海洋湖沼通报》2024年第2期134-142,共9页Transactions of Oceanology and Limnology

基　　金：广东省对外科技合作项目(2013B051000081);广东省大学生创新创业训练计划项目(CXXL2018051);广东省自然科学基金项目(2018A030313212)。

摘　　要：为探讨海水中铜离子(Cu^(2+))污染对喜盐草(Halophila ovalis)生长的影响,通过室内种植喜盐草,然后添加不同质量浓度重金属Cu^(2+),培养2周后测定了喜盐草生物量、色素含量、可溶性蛋白含量、3种抗氧化酶活性及植物组织内的铜含量。结果显示:不同质量浓度的Cu^(2+)对植株的生物量和相对生长速率影响显著(P<0.05),对色素含量的影响不显著(P>0.05);可溶性蛋白含量随着Cu^(2+)质量浓度的增加而增加;超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性随着Cu^(2+)质量浓度的增加而降低;喜盐草地上部分对重金属铜富集量高于地下部分,且随着海水中Cu^(2+)含量的增加富集量增加。研究表明,当海水重金属Cu^(2+)质量浓度超过0.1 mg/L时,喜盐草生长速率、抗氧化酶活性显著降低,可导致海草床退化;重金属铜主要富集于地上部分,可导致喜盐草快速落叶,影响海草床的生态功能。To determine the effect of seawater bivalent copper ion(Cu^(2+))at different concentrations on the growth of Halophila ovalis,seagrass H.oualis was planted in door and in seawater containing different amounts of Cu^(2+).After 2 weeks culture,the biomass,pigment content,soluble protein content,three antioxidant enzyme activities of H.ovalis and copper content in its tissues were measured.The results showed that the increase of Cu^(2+)content significantly affected the biomass and relative growth rate(RGR)(P<0.05).The content of Cu^(2+)had no significant effect on the pigment content(P>0.05).The soluble protein content increased with the increase of Cu^(2+)content.The activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)decreased with the increase of Cu^(2+)content.The amount of copper in above ground tssues was higher than that in underground parts.Moreover,with the increase of Cu^(2+)content in seawater,more copper was enriched in plant tissues.We found also that the growth rate and the antioxidant enzyme activities of H.ovalis were decreased significantly when the Cu^(2+)was high than O.1 mg/L in seawater.The high concentrations of Cu^(2+)caused the degradation of seagrass bed.Copper was enriched in the leaves of H.ovualis,leading to the rapid defoliation of H.oualis and affecting the ecological function of seagrass bed.

关 键 词：喜盐草 铜 生长 抗氧化酶 富集 

分 类 号：Q945.78[生物学—植物学]