云南省洱源县人群芽囊原虫和卡耶塔环孢子虫感染情况及其分子特征  

Infection of Blastocystis and Cyclospora cayetanensis in residents in Eryuan County,Yunnan Province and their molecular characterization

作　　者：王雅雪 简金花 刘华 秦源 彭晓雪 苏雅馨 沈玉娟 WANG Yaxue;JIAN Jinhua;LIU Hua;QIN Yuan;PENG Xiaoxue;SU Yaxin;SHEN Yujuan(National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Chinese Center for Tropical Diseases Research,National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,NHC Key Laboratory of Parasite and Vector Biology,WHO Collaborating Centre for Tropical Diseases,National Center for International Research on Tropical Diseases,Shanghai 200025,China;Hangzhou Medical College,Hangzhou 310000,Zhejiang,China;School of Global Health,Chinese Center for Tropical Diseases Research,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China)

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所(国家热带病研究中心),传染病溯源预警与智能决策全国重点实验室,国家卫健委寄生虫病原与媒介生物学重点实验室,世界卫生组织热带病合作中心,国家级热带病国际联合中心,上海200025 [2]杭州医学院,浙江杭州310000 [3]上海交通大学医学院-国家热带病研究中心全球健康学院,上海200025

出　　处：《中国寄生虫学与寄生虫病杂志》2024年第2期242-250,共9页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金(82372283,82072307);上海市加强公共卫生体系建设三年行动计划(2023—2025)重点学科项目(GWVI-11.1-09)。

摘　　要：目的了解云南省洱源县人群芽囊原虫和卡耶塔环孢子虫的感染情况及其分子特征,为寄生虫病防治工作提供参考。方法2022年7—8月在云南省洱源县5个村庄采用简单随机抽样法抽取1岁以上的常住居民作为调查对象,采集调查对象新鲜粪样,收集其社会人口学信息。提取粪样DNA,使用普通PCR和巢式PCR分别扩增芽囊原虫和卡耶塔环孢子虫的核糖体小亚基RNA(SSU rRNA)基因,分析人群感染情况。对阳性PCR扩增产物进行双向测序,在NCBI进行BLAST比对分析,鉴定虫种和基因亚型,采用MEGA 11.0软件以邻接法构建系统进化树。用SPSS 21.0软件进行统计学分析,感染率的比较采用χ2检验或Fisher确切概率法。结果共采集100份居民粪样。芽囊原虫感染率为14.0%(14/100)。其中,男性、女性芽囊原虫感染率分别为14.6%(6/41)和13.6%(8/59);60岁以上的居民和家庭人口数为6人及以上的感染率最高,分别为18.5%(5/27)、3/15;未接受过教育和小学文化者感染率较高,分别为2/11和17.0%(8/47);感染者均为农民。不同性别、年龄、家庭人口数、教育水平和职业等人群的芽囊原虫感染率差异均无统计学意义(χ2=0.023、2.730、2.235、1.404、1.668,均P>0.05)。卡耶塔环孢子虫感染率为2.0%(2/100),感染者均为女性农民。未查见芽囊原虫和卡耶塔环孢子虫的混合感染。14份芽囊原虫阳性样本中扩增出ST1和ST3亚型序列各7条,其中7条ST1序列有25个核苷酸差异,5条与芽囊原虫(GenBank:ON932511、KU147348、MK801408、MW728079、OR754904)的序列一致性分别为100%,2条为新序列(GenBank:PP439288、PP439289),与芽囊原虫(GenBank:OP725964、KU147333)一致性为99.8%;7条ST3序列有1个核苷酸差异,与芽囊原虫(GenBank:KU147372、MK801366)的序列一致性分别为100%。扩增获得的2条卡耶塔环孢子虫序列相同,与卡耶塔环孢子虫(GenBank:KY770755)的序列一致性为100%。系统进化树显示,本研究中芽囊�Objective To investigate the prevalence and molecular characterizations of Blastocystis and Cy⁃clospora cayetanensis in residents in Eryuan County,Yunnan Province to provide reference for parasitic disease control and prevention.Methods From July to August 2022,permanent residents over the age of 1 were enrolled for the survey by simple random sampling from the five villages in Eryuan County,Yunnan Province.Fresh fecal samples and their social demographic information were collected.The fecal genomic DNA was extracted,and the ribosomal small subunit RNA(SSU rRNA)genes of Blastocystis and C.cayetanensis were amplified by PCR and nested PCR,respectively,in order to analyze the prevalence in the residents.The positive products were sequenced bidirectionally,the species and gene subtypes were identified by BLAST alignment in NCBI.The phylogenetic trees were constructed using the neighbor-Joining method by MEGA 11.0 software.SPSS 21.0 software was used for statistical analysis,and the infection rates were compared byχ2 test or Fisher’s exact probability method.Results A total of 100 fecal samples were obtained from residents,of which the Blastocystis infection rate was 14.0%(14/100).Among them,the Blastocystis infection rates in male and female were 14.6%(6/41)and 13.6%(8/59),respectively.Residents over 60 years old and the family size of 6 persons and above had the highest infection rate,which was 18.5%(5/27)and 3/15,respectively.The infection rates were 2/11 and 17.0%(8/47)for those with no education and only elementary school education,respectively.All the infected residents were farmers.There was no statistically significant difference in Blasto⁃cystis infection rate by gender,age,family size,education level and occupation(χ2=0.023,2.730,2.235,1.404,1.668;all P>0.05).The C.cayetanensis infection rate was 2.0%(2/100).All of the infected residents were female farmers.No mixed infection of Blastocystis and C.cayetanensis was detected.Both 7 ST1 and ST3 sequences were amplified from 14 Blastocystis-positive sam

关 键 词：芽囊原虫 卡耶塔环孢子虫 感染 分子特征 洱源县 

分 类 号：R531.9[医药卫生—内科学]