急性下呼吸道感染患儿支气管肺泡灌洗液病原菌特征分析  

Pathogenic bacteria characteristics of bronchoalveolar lavage fluid in children with acute lower respiratory tract infection

作　　者：张炜煜[1] 柳鸿敏[1] 杨显达[1] 张立夫[1] 姚佳彤 李静[1] ZHANG Weiju;LIU Hongmin;YANG Xianda;ZHANG Lifu;YAO Jiatong;LI Jing(Center for Disease Control and Preven tion of Jilin Province,Jilin Changchang 130062,China)

机构地区：[1]吉林省疾病预防控制中心(吉林省公共卫生研究院),吉林长春130062

出　　处：《中国实验诊断学》2024年第5期505-509,共5页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省卫生健康技术创新项目(2018J035)/吉林省卫生健康技术创新项目(2018Q050)。

摘　　要：目的研究急性下呼吸道感染患儿病原菌特点,探讨支气管肺泡灌洗液荧光PCR检测对儿童急性呼吸道感染病原学的诊断价值。方法选择2018年6月至2019年5月吉林大学第一医院收治的急性呼吸道感染住院患儿172例为研究对象,收集其支气管肺泡灌洗液,通过多重实时荧光定量PCR检测其病原体。结果本研究中172例支气管肺泡灌洗液样本致病菌检出有106例,阳性率为61.63%(106/172);排在前三位的病原菌检出率依次为肺炎链球菌26.74%(46/172),流感嗜血杆菌17.44%(30/172),百日咳鲍特菌8.14%(14/172),且不同年龄组的病原菌检出率存在差异,P<0.01。结论急性下呼吸道感染患儿支气管肺泡灌洗液中肺炎链球菌检出率最高,依次为流感嗜血杆菌、百日咳鲍特菌、卡他莫兰汉菌、金黄色葡萄球菌。急性下呼吸道感染患儿的病原菌检出率在不同年龄段分组中存在差异。Objective To study the characteristics of pathogenic bacteria in children with acute respiratory tract in-fection,and to explore the diagnostic value of real-time PCR detection of bronchoalveolar lavage fluid samples from chil-dren with acute lower respiratory tract infections.Methods A total of 172 hospitalized children with acute respiratory tract infection admitted to the First Hospital of Jilin University from June 2018 to May 2019 were selected as the study objects.Bronchoalveolar lavage fluid was collected and pathogens were detected by multiple real-time PCR.Results In this study,106 Bronchoalveolar lavage samples were detected,the positive rate was 61.63%(106172),the top three pathogens were Streptococcus pneumoniae 26.74%(46172),Haemophilus influenzae 17.44%(30172),the detection rate of bordetella pertussis was 8.14%(14/172),and there was difference among different age groups(P<0.01).Con-clusion The detection rate of Streptococcus pneumoniae was the highest in children with acute lower respiratory tract infection,followed by Haemophilus influenzae,bordetella pertussis and Staphylococcus aureus.The detection rate of pathogenic bacteria in children with acute lower respiratory tract infection was different in different age groups.

关 键 词：呼吸道病原菌、支气管肺泡灌洗液 下呼吸道感染 实时荧光定量PCR 

分 类 号：R725.6[医药卫生—儿科]