禽多病因气囊炎病原的分离鉴定及耐药性分析  

Isolation,Identification and Antibiotic Resistance of Pathogen Isolated from Avian with Multicausal Airsacculitis

作　　者：韩倩 尤欣 郭思桃 姜瑞丽 钱雪桥 王永强 刘佳旭 HAN Qian;YOU Xin;GUO Si-tao(Guangdong HAID Group Co.Ltd.,Key Laboratory of Microecological Resources and Utilization in Breeding Industry,Ministry of Agriculture and Rural Affairs,Guangzhou,Guangdong 511400)

机构地区：[1]广东海大集团股份有限公司农业农村部微生态资源养殖利用重点实验室,广东广州511400

出　　处：《安徽农业科学》2024年第11期85-88,共4页Journal of Anhui Agricultural Sciences

基　　金：广州市高水平企业研究院项目(202205110006)。

摘　　要：为了解广东省四会地区某鸡场多病因气囊炎的主要致病菌及其耐药情况。采用形态学鉴定、16S rRNA基因测序等方法对分离株进行鉴定,选取3种常用抗生素进行药物敏感性试验,进行耐药性研究。结果表明:第1株分离株菌落呈典型的“荷包蛋”状,第2株分离株为革兰氏阴性杆菌,第3株分离株为革兰氏阳性球菌,经16S rRNA基因测序比对分析,3种病原的同源性均在99%以上,确定多病因气囊炎的主要致病菌为鸡毒支原体、大肠埃希氏杆菌和金黄色葡萄球菌,分别命名为SH-MG株、SH-E株和SH-S株。药物敏感性结果表明,SH-E株对阿莫西林、氟苯尼考和泰妙菌素产生了耐药性,SH-S株对阿莫西林产生了耐药性,SH-MG株对泰妙菌素敏感。说明禽多病因气囊炎很可能是金黄色葡萄球菌、大肠杆菌和鸡毒支原体共同感染造成的,其中大肠杆菌和金黄色葡萄球菌对一些常用的抗生素表现出耐药性,泰妙菌素是防治鸡毒支原体感染的首选药物。In order to investigate the major pathogen of multicausal airsacculitis and its antibiotic resistance in a hennery in Sihui area of Guangdong Province,the morphological identification,16S rRNA gene sequencing were performed,and drug resistance of these isolates were assessed using MIC.The results showed that the colonies of the first isolate had a typical“poached egg”shape,the second isolate was Gram-negative bacilli and the third isolate was Gram-positive cocci.16S rRNA gene sequencing analysis results showed that the isolated strains were identified as Mycoplasma gallinarum,Escherichia coli and Staphylococcus aureus,named as SH-MG strain,SH-E strain and SH-S strain,respectively.Drug resistance results showed that SH-E strain was resistant to amoxicillin,florfenicol and tiamulin,and SH-S strain was resistant to amoxicillin.We conclude that the multicausal airsacculitis is probably caused by co-infection of drug-resistant Staphylococcus aureus,drug-resistant Escherichia coli and Mycoplasma gallinarum,tiamulin is the first choice for the prevention and treatment of mycoplasma gallinis infection.

关 键 词：禽多病因气囊炎 分离鉴定 耐药性 鸡毒支原体 禽致病性大肠杆菌 金黄色葡萄球菌 

分 类 号：S858.31[农业科学—临床兽医学]