不同潮汐环境鱼藤幼苗叶片抗氧化酶活性的生理响应  

Physiological responses of antioxidant enzymes in the leaves of Derris trifoliata seedlings under varying tidal conditions

作　　者：吕晓波 李东海[2] 李剑碧 LYU Xiaobo;LI Donghai;LI Jianbi(Hainan Academy of Forestry/Hainan Academy of Mangroves,Haikou,Hainan 571100,China;College of Ecology and Environment,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南省林业科学研究院/海南省红树林研究院,海口571100 [2]海南大学生态与环境学院,海口570228

出　　处：《热带生物学报》2024年第3期281-289,共9页Journal of Tropical Biology

基　　金：海南省林业科学研究院科技创新基金项目(CXJJ-2020-001)。

摘　　要：为了探讨鱼藤(Derris trifoliata)幼苗在不同的水盐环境中的生理生化过程的变化,通过人工控制实验模拟25种潮汐环境,培育鱼藤幼苗,观测其在不同潮汐环境中,叶片内抗氧化酶活性、渗透调节物和膜系统损伤程度。结果表明:在盐度和淹水单一因素影响下,在盐度0~40范围内,鱼藤幼苗叶片内的SOD和CAT活性均呈现先升后降的变化趋势,在盐度20环境中出现峰值;在淹水时间0~8 h·d^(-1)范围内,鱼藤幼苗叶片内的SOD和CAT活性均变化不显著。鱼藤幼苗叶片内游离脯氨酸FPRO和丙二醛(MDA)的含量,随着盐度和淹水时间的增加呈现增长的变化趋势,分别在盐度40和淹水8 h·d^(-1)的处理中达到最大。在本研究设定的盐度和淹水时间共同作用下,鱼藤幼苗叶片内的SOD活性、CAT活性、FPRO和MDA含量分别在:盐度20+淹水6 h·d^(-1)、盐度20+淹水8 h·d^(-1)、盐度40+淹水8 h·d^(-1)的环境中出现峰值。综上所述,鱼藤幼苗在淹水时间短、盐度低的环境中,其抗氧化酶抵御胁迫损伤的能力较强,生长较正常,因此,对该环境中的红树林植被影响相对较大,而在淹水时间长、盐度高的环境中,抗氧化酶抵御胁迫损伤的能力较弱,生长受到抑制,因此对该条件下的红树林植被影响相对减小。In recent years the rapid proliferation of Derris trifoliata populations in mangrove areas has resulted in extensive mortality of mangrove plants in certain regions,posing a serious threat to the mangrove wetland ecosystem.During field investigations it was observed that the impact of D.trifoliata on mangrove plants was more pronounced in areas with shorter flooding durations and lower salinity levels compared to those with longer flooding durations and higher salinity levels.To investigate the underlying causes of this phenomenon,an attempt was made to simulate 25 different tidal environments through controlled artificial experiments,under which D.trifoliata seedlings were raised to observe variations in antioxidant enzyme activity,osmotic regulation mechanisms,and membrane system damage levels in their leaves.The results demonstrated that under the individual influence of salinity and flood,the activities of superoxide dismutase(SOD)and catalase(CAT)in the leaves of D.trifoliata seedlings initially increased and then decreased at a salinity level of 0-40‰.The peak value was observed at a salinity level of 20‰.In D.trifoliata seedlings SOD and CAT activities in the leaves did not show significant changes during the 0 h/d-8 h/d flooding period.However,free proline(FPRO)and malondialdehyde(MDA)contents in the seedling leaves exhibited an increasing trend with salinity levels and flooding duration,and their maximum values were reached under treatment conditions of 40‰salinity and 8 h/d flooding.When both salinity levels and flooding duration were put together,the SOD activity,CAT activity,FPRO content,and MDA content in the seedling leaves came to peak under the treatment of 20‰salt for 6 h/d,20‰salt for 8 h/d,and 40‰salt for 8 h/d,respectively.In summary,in an environment with short flooding durations and low salinity levels,D.trifoliata seedlings exhibited a heightened capacity for antioxidant enzymes to combat stress-induced damage,resulting in normal growth and consequently a relatively significant

关 键 词：红树林 鱼藤 生理胁迫 生理适应性 

分 类 号：S765[农业科学—森林保护学]