切段部位、长度和培养温度对琼枝藻繁殖的影响  

Effects of explant position,section length and culture temperature on the propagation of Betaphycus gelatinae

作　　者：童立豪 黄良夫 吴翔宇 石耀华[3] 唐贤明 TONG Lihao;HUANG Liangfu;WU Xiangyu;SHI Yaohua;TANG Xianming(Hainan Provincial Key Laboratory of Tropical Maricultural Technologies/Hainan Academy of Ocean and Fisheries Sciences,Haikou,Hainan 571126,China;Guangxi Key Lab of Mangrove Conservation and Utilization/Guangxi Academy of Marine Sciences(Guangxi Mangrove Research Center)/Guangxi Academy of Sciences,Beihai,Guangxi 536007,China;Ocean College,Hainan University,Haikou Hainan 570228,China)

机构地区：[1]海南省海洋与渔业科学院/海南省热带海水养殖技术重点实验室,海口571126 [2]广西科学院广西海洋科学院(广西红树林研究中心)/广西红树林保护与利用重点实验室,广西北海536007 [3]海南大学海洋学院,海口570228

出　　处：《热带生物学报》2024年第3期361-367,共7页Journal of Tropical Biology

基　　金：海南省自然科学基金项目(321QN367);国家藻类产业技术体系(CARS-50);海南省重点研发项目(ZDYF2020176)。

摘　　要：为了探究琼枝藻(Betaphycus gelatinae)组织切段培养的适宜条件,以琼枝藻为材料,研究不同切段部位(茎尖、分枝中部和主干)和切段长度(5、10、15 mm)的外植体切段在不同培养温度(21、24、27、30、33℃)下的出芽和生长。不同切段部位和切段长度的成活率和出芽率均达100%。不同切段部位出芽数由大到小依次为主干、分枝中部和茎尖;茎尖以伸长生长为主,主干和分枝中部则以上端切面膨大发芽为主;芽体形成于上端切面皮层,下端切面无芽体生成,表现出明显的极性。不同切段长度出芽数由大到小依次为15 mm组、10 mm组和5 mm组,15 mm组的个体出芽数分别为茎尖(5.90±0.67)个、分枝中部(10.16±1.14)个、主干(11.90±0.88)个;5 mm组出芽密度最高。琼枝藻切段培养适宜的生长温度为(21~27)℃,在此范围内,成活率和出芽率均为100%,且出芽数随温度的升高而增加;超过27℃,藻体切段的成活率和出芽率均下降。研究结果表明,琼枝藻最适组织切段培养部位为主干,长度为15 mm,培养温度为27℃。Betaphycus gelatinae was cut into sections of different lengths from different explants and cultured at different temperatures to explore the suitable conditions for its tissue culture to determine the effects of different explants(stem tip,main branch and trunk),section lengths(5 mm,10 mm and 15 mm)and culture temperatures(21℃,24℃,27℃,30℃and 33℃)on bud emergence and growth of the explants of B.gelatinae.The results showed that the survival rate and bud emergence rate of different explants and section lengths were all up to 100%.The number of buds in different explants was in the order of the trunk>the main branch>the stem tip.The stem tip mainly grew elongate,while the main branch and trunk were mainly expanded and formed buds.The buds were formed on the upper cut,not on the lower cut,showing obvious polarity.The number of the buds in the sections of different lengths was in the order of the sections 15 mm long>the sections 10 mm long>the sections 5 mm long.The number of individual buds in the sections 15 mm long was 5.90±0.67 in the stem tip,10.16±1.14 in the main branch and 11.90±0.88 in the trunk.The highest budding density was in the sections 5 mm long.The suitable growth temperature for tissue culture of B.gelatinae ranged from 21℃to 27℃,under which the survival rate and bud emergence rate were both 100%,and the number of buds increased with the temperature.The survival rate and bud emergenc rate of the sections were decreased when the temperature was above 27℃.For optimal tissue culture B.gelatinae was cultured at 27℃with the trunk sections 15 mm long as explants.

关 键 词：琼枝藻 外植体 组织培养 出芽增殖 温度 

分 类 号：S917.3[农业科学—水产科学]