瘤背石磺的化学成分研究  

Analysis of chemical constituents of extracts from Onchidium verruculatum

作　　者：邓雨婷 黄兹宝 刘雪菲丹 曹明玉 周媛媛 董琳[1] 张小坡[1] DENG Yuting;HUANG Zibao;LIU Xuefeidan;CAO Mingyu;ZHOU Yuanyuan;DONG Lin;ZHANG Xiaopo(Pharmacy College,Hainan Medical University,Haikou,Hainan 571199,China)

机构地区：[1]海南医学院药学院,海口571199

出　　处：《热带生物学报》2024年第3期368-372,共5页Journal of Tropical Biology

基　　金：海南省重点研究发展计划(ZDYF2022SHFZ037);国家自然科学基金项目(82060778)。

摘　　要：采用多种分离纯化方法,包括硅胶、凝胶、薄层色谱以及半制备高效液相等;并结合瘤背石磺生化特性、NMR数据分析及文献比较,对化合物进行结构鉴定。从瘤背石磺中分离鉴定出12个化合物,经鉴定结构为Cyclo-(L-Pro-L-Val)(1)、Cyclo-(Pro-Ile)(2)、Cyclo-(L-Pro-D-Leu)(3)、Tyramine(4)、Cyclo-(L-ProL-Phe)(5)、2-哌啶酮(6)、Cyclo-(L-Ala-L-Pro)(7)、尿嘧啶核糖核苷(8)、尿嘧啶(9)、胸腺嘧啶(10)、2'-甲氧基尿嘧啶核苷(11)、胸苷(12)。化合物(11~6,11)均为首次从瘤背石磺中分离得到。Onchidium verruculatum,a characteristic resource in the South China Sea,was extracted and isolated by using a variety of separation and purification methods,including silica gel,Sephadex LH-20,thin layer chromatography and semi-prepared HPLC.The structures of the compounds were identified by combining their biochemical characteristics,NMR data analysis and reference comparison.Twelve compounds were isolated from Onchidium verruculatum,and their structures were identified as Cyclo-(L-Pro-L-Val)(1),Cyclo-(Pro-Ile)(2),Cyclo-(L-Pro-D-Leu)(3),Tyramine(4),Cyclo-(L-Pro-L-Phe)(5),2-Piperidone(6),Cyclo-(L-Ala-L-Pro)(7),uracil ribonucleoside(8),uracil(9),thymine(10),2'-methoxyuracil nucleoside(11),thymidine(12).Compounds(1-6,11)were isolated for the first time from Onchidium verruculatum.

关 键 词：瘤背石磺 化学成分 高效液相色谱 

分 类 号：R931.77[医药卫生—生药学]