癃清胶囊抗尿路感染临床研究及网络药理学作用机制探索  

Clinical Study and Network Pharmacological Mechanism Exploration of Longqing Capsulein Treating Urinary Tract Infection

作　　者：冉超 罗庆林 侯瑶 张焱[2] 沈霖[3] RAN Chao;LUO Qinglin;HOU Yao;ZHANG Yan;SHEN Lin(Chongqing Kerui Nanhai Pharmaceutical Co.LTD.,Chongqing 409006,China;School of Pharmacy,Chongqing University,Chongqing 401331,China;Department of Integrated Traditional Chinese and Western Medicine,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China)

机构地区：[1]重庆科瑞南海制药有限责任公司,重庆409006 [2]重庆大学药学院,重庆401331 [3]华中科技大学同济医学院附属协和医院中西医结合科,武汉430022

出　　处：《中西医结合研究》2024年第3期145-154,159,共11页Research of Integrated Traditional Chinese and Western Medicine

摘　　要：目的通过临床研究和网络药理学探析癃清胶囊抗尿路感染的疗效和机制。方法选取西医确诊为单纯性尿路感染,且中医辨证为下焦湿热证的女性患者60例,将其随机分为对照组和试验组,每组30例。2组在均给予头孢克洛口服治疗基础上,试验组口服癃清胶囊,对照组口服癃清胶囊模拟剂。治疗7 d后,比较2组临床症状评分、生活质量评分、单项症状消失时间、总有效率及安全性指标。利用TCMSP和Swiss Target Prediction数据库获取癃清胶囊的活性成分及潜在作用靶点,通过GeneCards数据库获取尿路感染的相关靶点,筛选共同靶点,运用Cytoscape构建“活性成分-靶点”网络;通过STRING数据库构建PPI网络并通过DAVID数据库进行KEGG和GO分析。结果与对照组相比,试验组尿急症状评分、生活质量评分及单项症状消失时间均降低(P均<0.05),治疗总有效率提高(P<0.05)。网络药理学结果显示槲皮素、山柰酚等是癃清胶囊抗尿路感染的主要有效成分,SRC、PIK3R1、HSP90AA1等基因为其抗尿路感染的核心靶点,且主要富集在癌症相关通路、MAPK等信号通路上。结论癃清胶囊可能通过癌症相关通路等信号通路发挥抗尿路感染作用,可改善尿频、尿急、尿痛等症状,提高生活质量。Objective To explore the therapeutic effect and mechanism of Longqing capsule in treating urinary tract infection through clinical study and network pharmacology.Methods Sixty female patients who were diagnosed as simple urinary tract infection by western medicine and identified as dampness-heat in lower jiao syndrome by traditional Chinese medicine were selected and randomly divided into the control group and the trial group,with 30 cases in each group.Both groups were given oral treatment with cefaclor.The trial group received oral administration of Longqing capsule,while the control group received oral administration of Longqing capsule mimetics.After 7 days of treatment,the clinical symptom scores,quality of life scores,disappearance time of individual symptoms,total effective rate and safety indicators were compared in both groups.At the same time,the TCMSP database and Swiss Target Prediction server were used to obtain the active ingredients and potential targets of Longqing capsule,and the GeneCards database was used to obtain the relevant targets of urinary tract infection.And the common targets were screened out,so as to construct the network of active ingredients-targets using Cytoscape.The PPI networks were constructed via the STRING database and KEGG and GO analyses were constructed via the DAVID database.Results Compared with the control group,the urinary urgency syndrome scores,quality of life scores and disappearance time of individual symptoms in the trial group were decreased(all P<0.05),while the total effective rate was increased(P<0.05).The network pharmacology results showed that quercetin,kaempferol and other active ingredients were the main active ingredients of Longqing capsule against urinary tract infection.SRC,PIK3R1,HSP90AA1 and other genes were the core targets of Longqing capsule against urinary tract infection,which mainly enriched in cancer related pathways,MAPK signaling pathways,and other signaling pathways.Conclusion Longqing capsule may exert its anti-urinary tract infection ef

关 键 词：癃清胶囊 临床研究 尿路感染 网络药理学 作用机制 

分 类 号：R277.5[医药卫生—中医学]