利拉鲁肽对高糖环境下牙周膜成纤维细胞迁移、增殖和炎症反应的影响  

Effect of liraglutide on migration,proliferation,and inflammatory response of periodontal ligament fibroblasts under high glucose environment

作　　者：崔丽娟 李桂平 CUI Li-juan;LI Gui-ping(Department of Endocrinology,Affiliated Huizhou Hospital,Guangzhou Medical University,Huizhou 516000,Guangdong,China)

机构地区：[1]广州医科大学附属惠州医院内分泌科,广东惠州516000

出　　处：《广东医学》2024年第4期433-438,共6页Guangdong Medical Journal

基　　金：广东省医学科学技术研究项目(A2019175)。

摘　　要：目的探讨利拉鲁肽对高糖环境下牙周膜成纤维细胞(PDLF)迁移、增殖和炎症反应的影响。方法体外培养牙周膜成纤维细胞,根据培养液成分设置为低糖对照组(L组,含5.5 mmol/L葡萄糖的DMEM培养基)、高糖组(H组,25 mmol/L葡萄糖的DMEM培养基)、低糖给药组(L+G组,10×10^(-7)mmol/L的利拉鲁肽+含5.5 mmol/L葡萄糖的DMEM培养基)和高糖给药组(H+G组,10×10^(-7)mmol/L的利拉鲁肽+25 mmol/L葡萄糖的DMEM培养基)。采用CCK-8法测定细胞增殖能力,Transwell小室测定细胞迁移能力,应用流式细胞仪测定细胞凋亡情况,采用Western blot法测定牙周膜成纤维细胞中Bax、Bcl-2表达水平,采用酶联免疫吸附法检测牙周膜成纤维细胞炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-6、C反应蛋白(CRP)的表达水平。结果与L组相比,H组PDLF细胞OD值、Transwell小室PDLF迁移数量、胞内Bcl-2蛋白水平降低(P<0.05);细胞凋亡率、胞内Bax蛋白、培养液中TNF-α、IL-1β、IL-6、CRP水平升高(P<0.05)。H+G组PDLF细胞OD值、Transwell小室PDLF迁移细胞数量、胞内Bcl-2蛋白水平显著高于H组(P<0.05);而细胞凋亡率、胞内Bax蛋白、培养液中TNF-α、IL-1β、IL-6、CRP水平则低于H组(P<0.05)。结论利拉鲁肽能够抑制高糖诱导的牙周膜成纤维细胞凋亡,通过调控Bax/Bcl-2表达和降低炎症反应,从而促进牙周组织愈合修复。Objective To investigate the effect of liraglutide on migration,proliferation,and inflammatory response of periodontal ligament fibroblasts(PDLF)under high glucose conditions.Methods PDLFs were cultured in vitro,and the culture medium was divided into low glucose control group(L group,DMEM medium containing 5.5 mmol/L glucose),high glucose group(H group,DMEM medium containing 25 mmol/L glucose),low glucose with liraglutide group(L+G group,DMEM medium containing 10×10^(-7)mmol/L liraglutide and 5.5 mmol/L glucose),and high glucose with liraglutide group(H+G group,DMEM medium containing 10×10^(-7)mmol/L liraglutide and 25 mmol/L glucose).Cell proliferation ability was determined using the CCK-8 method,cell migration ability was assessed using Transwell chambers,and apoptosis was measured by flow cytometry.The expression levels of Bax and Bcl-2 in PDLFs were detected by Western blot,and the expression of inflammatory factors tumor necrosis factor-α(TNF-α),interleukin(IL)-1β,IL-6,and C reactive protein(CRP)in PDLFs was determined by enzyme-linked immunosorbent assay.Results Compared with the L group,the OD value,migration quantity,and intracellular Bcl-2 protein level of PDLFs in the H group decreased(P<0.05);the apoptosis rate,intracellular Bax protein,and levels of TNF-α,IL-1β,IL-6,and CRP in the culture medium increased(P<0.05).In the H+G group,the OD value,migration quantity,and intracellular Bcl-2 protein level of PDLFs were significantly higher than those in the H group(P<0.05);while the apoptosis rate,intracellular Bax protein,and levels of TNF-α,IL-1β,IL-6,and CRP in the culture medium were lower than those in the H group(P<0.05).Conclusion Liraglutide can inhibit high glucose-induced apoptosis of PDLFs,promote periodontal tissue healing and repair by regulating the expression of Bax/Bcl-2,and reducing the inflammatory response.

关 键 词：利拉鲁肽 高糖 牙周膜成纤维细胞 BAX/BCL-2 炎症反应 

分 类 号：R589.1[医药卫生—内分泌] R781.4[医药卫生—内科学]