稻田稗草种群对氰氟草酯的抗性水平和抗性机理初探  

A preliminary study on the resistance level and resistance mechanism to cyhalofop-butyl in Echinochloa crus-galli populations in rice field

作　　者：张思思 刘敏 方宇航 齐家乐 潘浪 ZHANG Sisi;LIU Min;FANG Yuhang;QI Jiale;PAN Lang(College of Plant Protection,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学植物保护学院,长沙410128

出　　处：《现代农药》2024年第3期17-22,共6页MODERN AGROCHEMICALS

摘　　要：为明确来自湖南和江西省稻田稗草种群对氰氟草酯的抗性水平和抗性机理,采用整株生物测定法、基因测序、代谢酶抑制剂联用试验进行了研究。结果显示:R1、R2、R3种群对氰氟草酯的抗性指数分别为3.08、2.19、3.83,均为中抗水平;R4种群抗性指数为1.45,为低抗水平。与敏感种群相比,R1种群存在Ile-2041-Val突变,R2种群中存在Ile-2041-Val、Asp-2078-Gly突变,且细胞色素P450酶抑制剂马拉硫磷和谷胱甘肽硫转移酶抑制剂NBD-Cl均能提高R1和R2种群对氰氟草酯的敏感性;R3种群中存在Trp-2027-Arg突变,且NBD-Cl可以提高其对氰氟草酯的敏感性;R4种群中存在Cys-2088-Tyr突变,马拉硫磷和NBD-Cl不能逆转其对氰氟草酯的抗性。研究表明,稗草种群R1、R2、R3对氰氟草酯的中等水平抗性是由靶标抗性和非靶标抗性共同介导,种群R4对氰氟草酯的低水平抗性主要是由靶标抗性介导。In order to clarify the resistance level and resistance mechanism to cyhalofop-butyl in Echinochloa crus-galli populations from Hunan and Jiangxi paddy fields,the experimental studies were carried out by whole-plant bioassay,gene sequencing,and metabolic enzyme inhibitor coupled with cyhalofop-butyl.The results showed that R1,R2 and R3 populations displayed medium level resistance to cyhalofop-butyl,with the resistance indexs of 3.08,2.19 and 3.83,respectively.R4 population displayed low level resistance to cyhalofop-butyl,with the resistance index of 1.45.The Ile-2041-Val mutation was present in R1,and the Ile-2041-Val and Asp-2078-Gly mutations were present in R2,and malathion,NBD-Cl increased the sensibilities of R1 and R2 to cyhalofop-butyl.The Trp-2027-Arg mutation was present in R3 and NBD-Cl could increase the sensitivity to cyhalofop-butyl.The Cys-2088-Tyr mutation was present in R4,malathion and NBD-Cl couldn't reverse the resistance to cyhalofop-butyl.This study indicated that the medium level resistance of R1,R2,and R3 to cyhalofop-butyl was caused by a combination of target-site and non-target-site resistance,the low level resistance of R4 to cyhalofop-butyl was mainly mediated by target-site resistance.

关 键 词：氰氟草酯 抗性水平 抗性机理 稗草 CYP450 GST 

分 类 号：TQ450.13[化学工程—农药化工]