高效液相色谱法测定高效氯氟氰菊酯微囊悬浮剂的优化研究  

Study on the optimization method for determination of lambda-cyhalothrin CS by HPLC

作　　者：王坤 吕霞 刘贝妮 吴晗 杨淑娴[1] WANG Kun;LV Xia;LIU Beini;WU Han;YANG Shuxian(Jiangsu Provincial Agro-product Supervision&Testing Center,Nanjing 210000,China)

机构地区：[1]江苏省农产品质量检验测试中心,南京210000

出　　处：《现代农药》2024年第3期57-61,共5页MODERN AGROCHEMICALS

摘　　要：在现有检测方法的基础上,研究了不同溶剂的破囊效果,建立了高效液相色谱法测定高效氯氟氰菊酯微囊悬浮剂中有效成分含量的方法。方法以乙醇为破囊溶剂,正己烷+乙醇为流动相,使用ZORBAX RX-SIL色谱柱和可变波长紫外检测器,在278 nm波长下对高效氯氟氰菊酯微囊悬浮剂进行分离和定量分析。结果表明,在质量浓度0.05~1.0 mg/mL时,方法线性关系良好,相关系数为0.9999;方法标准偏差为0.06,相对标准偏差为0.55%,平均回收率为100.81%。该分析方法操作简易、安全、准确,分离效果好,可用于高效氯氟氰菊酯微囊悬浮剂有效成分的分析检测。Based on existing methods,the destruction effect on microcapsule capsule wall using different solvents were investigated,and a separation and quantitative analysis method for lambda-cyhalothrin CS was established by HPLC,with ethanol as solvent,n-hexane and ethanol as mobile phase,on ZORBAX RX-SIL column and DAD at 278 nm wavelength.The results showed that the linear correlation coefficient was 0.9999 at the concentration of 0.05-1.0 mg/mL,the standard deviation was 0.06,the relative standard deviation was 0.55%,and the average recovery was 100.81%.The method had the advantages of rapidity,simple operation,accuracy and good separation effect.

关 键 词：高效氯氟氰菊酯 微囊悬浮剂 破囊 高效液相色谱 分析 

分 类 号：TQ450.7[化学工程—农药化工]