QuEChERS-超高效液相色谱-串联质谱法检测果蔬中ipfentrifluconazole残留量  

Determination of ipfentrifluconazole residues in fruits and vegetables by QuEChERS-UPLC-MS/MS

作　　者：骆玉琴 王卢燕 张昌朋[1] 何红梅[1] 王祥云[1] 赵学平[1] 蒋金花[1] LUO Yuqin;WANG Luyan;ZHANG Changpeng;HE Hongmei;WANG Xiangyun;ZHAO Xueping;JIANG Jinhua(State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Institute of Agro-product Safety and Nutrition,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]省部共建农产品质量安全危害因子与风险防控国家重点实验室,浙江省农业科学院农产品质量安全与营养研究所,杭州310021

出　　处：《现代农药》2024年第3期65-68,85,共5页MODERN AGROCHEMICALS

基　　金：国家自然科学基金面上项目(32072455)。

摘　　要：建立一种QuEChERS-超高效液相色谱-串联质谱法测定果蔬中ipfentrifluconazole残留的分析方法。样品经乙腈提取,无水硫酸镁、PSA、GCB净化,滤膜过滤后采用UPLC-MS/MS测定,外标法定量。结果表明,在质量浓度0.005~0.5 mg/L,方法线性关系良好,相关系数大于0.995,定量限均为0.01 mg/kg。在3个添加水平(0.01、0.1、0.5 mg/kg)下,ipfentrifluconazole在5种果蔬中的平均添加回收率为84.0%~102.1%,相对标准偏差为2.1%~12.7%。该方法简单、灵敏、高效,可用于果蔬中ipfentrifluconazole残留检测。A QuEChERS-ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method was developed for the determination of ipfentrifluconazole residues in fruits and vegetables.The samples were extracted by acetonitrile,purified by anhydrous magnesium sulfate,PSA and GCB,filtered by membrane,and then determined by UPLC-MS/MS,quantified by an external standard method.The results showed that the method had good linear relationship at the concentration of 0.005 to 0.5 mg/L,the correlation coefficients all over 0.995.The limits of quantification in five matrix were 0.01mg/kg.At the spiked levels of 0.01,0.1 and 0.5 mg/kg,the average recoveries of ipfentrifluconazole were 84.0%-102.1%,and the relative standard deviations were 2.1%-12.7%.The method was simple,sensitive and efficient,and could be used for ipfentrifluconazole residue detection in fruits and vegetables.

关 键 词：QUECHERS 超高效液相色谱-串联质谱 ipfentrifluconazole 果蔬 残留 检测 

分 类 号：TQ450.263[化学工程—农药化工]