丁酸梭菌对七彩神仙鱼生长、形体指标、营养利用和肠道结构功能的影响  

Effects of Clostridium butyricum on growth,body index,nutrient utilization,intestinal structure and function of discus fish(Symphysodon haraldi)

作　　者：朱建国 温彬 成果 高建忠[1,2,3,4] 陈再忠[1,2,3,4] ZHU Jianguo;WEN Bin;CHENG Guo;GAO Jianzhong;CHEN Zaizhong(Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China;Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai 201306,China;National Demonstration Centre for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学,农业农村部淡水水产种质资源重点实验室,上海201306 [2]上海海洋大学,水产种质资源发掘与利用教育部重点实验室,上海201306 [3]上海海洋大学,上海水产养殖工程技术研究中心,上海201306 [4]上海海洋大学,水产科学国家级实验教学示范中心,上海201306

出　　处：《水产学报》2024年第6期159-168,共10页Journal of Fisheries of China

基　　金：上海扬帆人才计划项目(19YF1419400);上海市自然科学基金(20ZR1423600)。

摘　　要：为探讨丁酸梭菌对七彩神仙鱼生长、形体指标、营养利用和肠道结构功能的影响,在基础饵料中分别添加0(对照)、1×10^(4)、1×10^(5)、1×10^(6)、1×10^(7)和1×10^(8) cfu/g丁酸梭菌饲养幼鱼8周。结果显示,与对照组相比,丁酸梭菌对七彩神仙鱼生长和形体指标无显著影响。1×10^(5) cfu/g添加组鱼体粗蛋白含量增加,对蛋白质的消化率显著提高。丁酸梭菌显著提高了中肠肠道绒毛高度和宽度,且1×105 cfu/g添加组肠道酸性黏多糖分泌量显著增加。对于肠道消化酶和抗氧化能力,1×10^(5) cfu/g添加组胰蛋白酶活性显著提高,1×10^(6) cfu/g添加组丙二醛含量显著降低。肠道内容物短链脂肪酸分析显示,1×10^(5) cfu/g添加组的乙酸和丙酸含量显著高于对照组,且总酸含量在该添加组达到最大,显著高于其他组。研究表明,在本试验条件下,七彩神仙鱼饵料中添加1×10^(5) cfu/g丁酸梭菌可以改善肠道结构,提高肠道消化和抗氧化能力,增加肠道短链脂肪酸的分泌和营养利用率。本研究可为丁酸梭菌在七彩神仙鱼饵料中的科学利用提供参考。To determine the effects of Clostridium butyricum on growth,body index,nutrient utilization,and intestinal structure and function of discus fish Symphysodon haraldi,C.butyricum was added in basic feed for the fish at concentrations of 0(control),1×10^(4),1×10^(5),1×10^(6),1×10^(7) and 1×10^(8) cfu/g,respectively.After 8-week culture,no significant difference was showed on growth and body index of fish in C.butyricum-added groups.However,crude protein content and digestibility in 1×10^(5) cfu/g group increased significantly.Compared to control,height and width of midgut villi in fish fed with C.butyricum was apparently increased and mucopolysaccharide secretion level of fish in 1×10^(5) cfu/g group significantly improved.For intestinal digestive and antioxidant enzymes,the trypsin activity of fish in 1×10^(5) cfu/g group was greatly improved.Furthermore,the malondialdehyde content in 1×10^(6) cfu/g group fish was significantly decreased.Short chain fatty acids(SCFAs)were measured in fish intestinal contents,and it was suggested that acetic acid and propionic acid in 1×10^(5) cfu/g group was significantly higher than that in the control group,and the total acid content reached the maximum in this addition group,which was significantly higher than other groups.Studies have shown that under the conditions of this experiment,adding 1×10^(5) cfu/g C.butyricum to the discus fish bait can improve intestinal structure,intestinal digestion and antioxidant capacity,and increase intestinal SCFAs secretion and nutrition utilization.

关 键 词：七彩神仙鱼 丁酸梭菌 生长 形体指标 营养利用 肠道结构功能 

分 类 号：S963.73[农业科学—水产养殖]