生姜组织培养和快繁技术研究  

Study on Tissue Culture and Rapid Propagation of Ginger

作　　者：王春勇 闫颖 李娟[1] 李贺 崔婧婧 刘鹏靖 韩靖玲[1] 张爱敏[1] WANG Chun-yong;YAN Ying;LI Juan;LI He;CUI Jing-jing;LIU Peng-jing;HAN Jing-ling;ZHANG Ai-min(Tangshan Academy of Agricultural Sciences,Tangshan 063000,China;Tangshan Institute of Landscape Sciences,Tangshan 063000,China)

机构地区：[1]唐山市农业科学研究院,河北唐山063000 [2]唐山园林科学研究所,河北唐山063000

出　　处：《河北农业科学》2024年第2期69-72,94,共5页Journal of Hebei Agricultural Sciences

基　　金：河北省植物生物技术研究与应用重点实验室(SZX2019018)。

摘　　要：以冀东地区传统主栽生姜品种“狮子头”的幼芽为外植体,通过对其组织培养(HgCl_(2)消毒时长,诱导培养基的6-BA浓度)和快速繁殖(增殖和生根同步进行的培养基激素浓度,移栽基质组分)中的主要关键技术进行研究,建立“狮子头”生姜的组培快繁技术体系。结果表明:外植体使用HgCl_(2)浸泡消毒的适宜时长为7 min,愈伤组织污染率为0;诱导培养基的6-BA适宜浓度为2.0 mg/L,不定芽诱导率为94%,污染率为0;实现增殖和生根同步培养的培养基6-BA适宜浓度为2.0 mg/L、NAA适宜浓度为0.2 mg/L,增殖倍数为5.14倍、生根数为0.99根/株;组培苗移栽到蛭石、草炭、泥沙按体积比1∶1∶1混合的基质上,植株长势强,成活率达到95%。“狮子头”生姜组培快繁的关键技术为:将姜芽用0.1%的HgCl_(2)溶液浸泡消毒7 min-将愈伤组织接种到MS+6-BA 3.0 mg/L+NAA 0.2 mg/L培养基上进行不定芽诱导—将不定芽接种到MS+6-BA 2.0 mg/L+NAA 0.2 mg/L培养基上进行增殖和生根的同步培养-将组培苗移栽到蛭石、草炭、泥沙按体积比1∶1∶1混合的基质上进行炼苗。The key technologies in tissue culture(HgCl_(2) disinfection time and 6-BA concentration in induced medium)and rapid propagation(hormone concentration in culture medium for simultaneous proliferation and rooting and transplantation substrate components)of the traditional main ginger variety Shizitou in the eastern Hebei region were studied,and the tissue culture rapid propagation technology system was established.The results showed that the suitable duration for soaking and disinfecting explants with HgCl_(2) was 7 minutes,and the contamination rate of callus tissue was 0.The suitable concentration of 6-BA for inducing culture medium was 2.0 mg/L,with an adventitious bud induction rate of 94%and a contamination rate of 0.The optimal concentration of 6-BA and NAA in the culture medium for simultaneous proliferation and rooting was 2.0 mg/L and 0.2 mg/L,respectively,the proliferation rate was 5.14 and the rooting number was 0.99 per plant.The tissue cultured seedlings transplanted on the substrate mixed with vermiculite,peat,and sediment in a volume ratio of 1∶1∶1 grew strongly with the survival rate of 95%.The key technology for rapid propagation of Shizitou ginger in tissue culture is:soaking and disinfecting ginger sprouts in 0.1%HgCl_(2) solution for 7 minutes-inoculating callus tissue on MS+6-BA 3.0 mg/L+NAA 0.2 mg/L medium for adventitious bud induction-inoculating adventitious buds on MS+6-BA 2.0 mg/L+NAA 0.2 mg/L medium for simultaneous proliferation and rooting-transplanting tissue culture seedlings onto a mixed substrate of vermiculite,peat,and sediment in a volume ratio of 1∶1∶1 for seedling refinement.

关 键 词：生姜 组织培养 激素浓度 培养基 快速繁殖 

分 类 号：S632.5[农业科学—蔬菜学]