红霉素菌渣厌氧消化产气潜能及动力学特性  

Methane production potential and kinetic characteristics of the anaerobic digestion of erythromycin fermentation residues

作　　者：任建军 毛洪刚 朱李霞 夏禧龙 周蓉蓉 韩丽丽 马娜 尹冬敏 REN Jianjun;MAO Honggang;ZHU Lixia;XIA Xilong;ZHOU Rongrong;HAN Lili;MA Na;YIN Dongmin(Changzhou Key Laboratory of Biomass Green,Safe&High Value Utilization,Institute of Urban and Rural Mining CCZU,Changzhou 213164,China;School of Pharmacy&School of Biological and Food Engineering,Changzhou University,Changzhou 213164,China;Beijing General Station of Animal Husbandry,Beijing 100107,China;Yili Keyuan Testing Technology Co.,Ltd,Yili 835000,China)

机构地区：[1]常州大学城乡矿山研究院常州市生物质绿色安全高值利用技术重点实验室,常州213164 [2]常州大学药学院生物与食品工程学院,常州213164 [3]北京市畜牧总站,北京100107 [4]伊犁科源检测技术有限公司,伊犁835000

出　　处：《农业工程学报》2024年第9期199-208,共10页Transactions of the Chinese Society of Agricultural Engineering

基　　金：常州市应用基础研究计划项目(CJ20235062);国家自然科学基金项目(32002210);江苏省高等学校基础科学(自然科学)研究重大项目(23KJA610001);贵州省科技厅科技支撑计划项目(黔科合支撑【2022】一般175);伊犁川宁生物技术有限公司合作开发项目(CN-ZH/2019-039)。

摘　　要：为探究未经脱抗预处理的抗生素菌渣作为单一底物厌氧无害化处理的可行性,该研究以新鲜的红霉素菌渣(erythromycin fermentation residue,EFR)及其上清液为原料,在常温(20℃)、中温(35℃)和高温(55℃)条件下开展产气潜能及其动力学研究。研究结果表明:中温条件下,EFR获得最高的累积甲烷产量,为460 mL/g,比常温和高温时分别提高了163%和55.5%。通过一级动力学模型可以看出,EFR的产甲烷过程呈现明显的两阶段特性,其中高温时的产甲烷速率(k1=0.1422 d^(−1))显著高于常温(k1=0.0429 d^(−1),P<0.05)和中温(k1=0.0363 d^(−1),P<0.05)。物料衡算分析显示,随着发酵温度的升高,EFR的水解转化率逐级提高,高温时最高为87%,表明高温条件下菌渣中残留的红霉素对水解菌基本未产生抑制作用。未经脱抗处理的EFR可直接作为厌氧发酵的原料,为菌渣的减量化和资源化处理提供了新的思路。Erythromycin fermentation residue(EFR)has been classified as a hazardous material,due to the high content of erythromycin and the spread of antibiotic-resistance genes.The removal of erythromycin from EFR can also be required for the hydrothermal pretreatment with the ultra-high temperature and high pressure for a long time(>160℃,>60 min),due to the stable 14-member-ring and the complex matric structure of mycelium.For instance,the spray drying flowered by incineration is expensive to afford,as well as organic wastes.Anaerobic digestion(AD)has been supposed as an alternative technology to reduce erythromycin with low energy consumption and bioenergy recovery.This study aims to investigate whether the EFR can be used as the substrate for AD without pretreatment.Methane potential experiments were then carried out via batch assays under different temperatures(20,35,and 55℃).EFR with liquid phase(named LEFR)was chosen as the raw material.Modified Gompertz model and the first-order kinetic model were applied to verify the capacity and efficiency of EFR and LEFR for methane production.The content of nitrogen in EFR was 8.1%(using day matter),according to the elemental analyzer and Buswell equation.In order to avoid the influence of ammonia inhibition,1 g of volatile solid(VS)of EFR or LEFR was added to each strum bottle.The highest concentration of ammonia nitrogen in theory was controlled within 1250 mg/L.The methane potential experiment showed that the highest accumulated biogas production was 675.2 mL/g(methane of 460 mL/g)obtained from EFR under mesophilic conditions,which was 163%and 55.5%higher than that under psychrophilic and thermophilic conditions,respectively.While thermophilic conditions showed that higher maximal methane yield rates(Rmax)were achieved in 32.30 and 11.70 mL/(g·d)for the EFR and LEFR than lower temperatures.In addition,the first-order kinetics of EFR presented outstandingly two-stage characteristics,compared with the LEFR.Among them,the first kinetics at 55℃(k1=0.1422 d-1 and k2=0.091

关 键 词：抗生素 菌渣 厌氧发酵 Gompertz模型 一级动力学 物料平衡 

分 类 号：S21[农业科学—农业机械化工程]