射干药效物质基础成分鸢尾黄素、鸢尾甲黄素A的pKa值测定  

Determination of pKa Value of Pharmacodynamic Substance Basis Tectorigenin and lristectorigenin A of Shegan(Belamcanda Chinensis)

作　　者：刘晶[1] 赵玥[1] 王光函[1] 张颖[1] 邸子真[1] 邹桂欣[1] LIU Jing;ZHAO Yue;WANG Guanghan;ZHANG Ying;DI Zizhen;ZOU Guixin(Liaoning Traditional Chinese Medicine Institute,Shenyang 110034,Liaoning,China)

机构地区：[1]辽宁省中医药研究院,辽宁沈阳110034

出　　处：《实用中医内科杂志》2024年第6期18-20,I0007,共4页Journal of Practical Traditional Chinese Internal Medicine

基　　金：科技部“十二五”科技重大专项(2017ZX09301019);辽宁省教育厅项目(L202031)。

摘　　要：目的 采用高效液相色谱法测定鸢尾黄素、鸢尾甲黄素A的pKa值,为其剂型设计提供参考。方法 采用乙腈-不同pH值磷酸缓冲盐水溶液为流动相,在检测波长265 nm下,依次测定鸢尾黄素、鸢尾甲黄素A的保留因子,从而计算pKa。结果 鸢尾黄素的pKa为6.49、鸢尾甲黄素A的pKa为6.51。结论 采用高效液相色谱法测定鸢尾黄素、鸢尾甲黄素A的pKa值,方法简便、有效。Objective To determine the pKa values of tectorigenin and iristectorigenin A by high performance liquid chromatog-raphy and provide reference for the formulation design.Methods The mobile phase was acetonitrile phosphoric acid buffer solution with different pH values.At the detection wavelength of 265 nm,the retention factors of tectorigenin and iristectorigenin A were determined successively and then the pKa value was calculated.Results The pKa values of tectorigenin was 6.49 and that of iris-tectorigenin A was 6.51.Conclusion High performance liquid chromatography is a simple and effective method for the determina-tion of pKa value of tectorigenin and iristectorigenin A.

关 键 词：鸢尾黄素 鸢尾甲黄素A PKA值 

分 类 号：R284.1[医药卫生—中药学]