基于网络药理学探究三七叶对糖尿病大鼠的治疗机制  

Anti-diabetic Activity of Sanqi(Panax notoginseng)Leaves Based on Network Pharmacology and Diabetic Rats

作　　者：于颖 王承潇[1,2] 杨野 崔秀明[1,2] YU Ying;WANG Chengxiao;YANG Ye;CUI Xiuming(School of Life Science and Technology,Kunming University of Technology,Kunming 650500,Yunnan,China;Key Laboratory of Panax notoginseng Resources Sustainable Development and Utilization,Kunming 650500,Yunnan,China)

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500 [2]云南省三七资源可持续利用重点实验室,云南昆明

出　　处：《实用中医内科杂志》2024年第6期21-24,I0008-I0011,共8页Journal of Practical Traditional Chinese Internal Medicine

基　　金：国家自然科学基金项目(82060645);云南省应用基础研究计划面上项目(202101AT070099);云南省科技重大专项(202102AA310045,202002AA1000056)。

摘　　要：目的 利用网络药理学和动物实验,研究三七叶抗糖尿病的主要活性成分和潜在作用机制。方法 基于Pubchem、PharmMapper、OMIM等数据库筛选三七叶与糖尿病共同的潜在靶点之后,再利用STRING数据库和Cytoscape软件构建蛋白相互作用网络图,对其进行拓扑学分析和聚类分析获得核心靶点,并进行GO和KEGG富集分析筛选药物作用于疾病的可能机制。随后进行动物实验验证三七叶的抗糖尿病作用。结果 预测结果共获得三七叶与糖尿病的共同靶点50个,核心靶点有3个:ANXA5、CFB、GSTP1,共涉及生物过程986种,细胞组成15种,分子功能74种,信号通路101条。动物实验表明三七叶可降低糖尿病大鼠的血糖,减少与糖尿病肾病相关的p38 MAPK和TGF-β1 mRNA的表达,同时也可以调节氧化应激和血脂水平,减少动脉粥样硬化。结论 三七叶是通过多途径多靶点共同发挥抗糖尿病的作用,其作用机制可能与减少氧化应激、调节细胞因子、减少动脉粥样硬化和抗糖尿病肾病肾纤维化等有关。Objective The main active ingredients and potential mechanisms of action of Sanqi(Panax notoginseng)leaves a-gainst diabetes were investigated using network pharmacology and animal experiments.Methods After screening the potential tar-gets shared by Sanqi(Panax notoginseng)leaves and diabetes based on Pubchem,PharmMapper and OMIM databases.The STRING database and Cytoscape software were used to construct protein interaction network maps.The topological analysis and cluster analysis were performed to obtain the core targets,and GO and KEGG enrichment analyses were performed to screen the possible mechanisms of drug action on the disease.Subsequently,animal experiments were conducted to verify the antidiabetic effect of Sanqi(Panax notoginseng)leaves.Results A total of 50 common targets of Sanqi(Panax notoginseng)leaves and diabe-tes were obtained,with three core targets:ANXA5,CFB and GSTP1,involving 986 biological processes,15 cellular compositions,74 molecular functions and 101 signaling pathways.Animal experiments have shown that Sanqi(Panax notoginseng)leaves can decrease blood glucose in diabetic rats,reduce the expressions of p38 MAPK and TGF-β1 mRNA associated with diabetic ne-phropathy,as well as regulate oxidative stress and lipid levels and reduce atherosclerosis.Conclusion Sanqi(Panax notoginseng)leaves exert its anti-diabetic effects through a combination of multiple pathways and targets,and its mechanism of action may be related to reducing oxidative stress,regulating cytokines,reducing atherosclerosis and anti-diabetic nephropathy renal fibrosis.

关 键 词：网络药理学 三七叶 糖尿病 信号通路 

分 类 号：R285[医药卫生—中药学] R96[医药卫生—中医学]