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作 者:方彦昊 王霄霄 魏亚琴[1] FANG Yanhao;WANG Xiaoxiao;WEI Yaqin(Anaerobic Microbiology Center,Institute of Biology,Gansu Academy of Sciences,Lanzhou,Gansu Province 730030,China;School of Life Science and Engineering,Lanzhou University of Technology,Lanzhou,Gansu Province 730050,China)
机构地区:[1]甘肃省科学院生物研究所厌氧微生物中心,甘肃兰州730030 [2]兰州理工大学生命科学与工程学院,甘肃兰州730050
出 处:《中国饲料》2024年第10期5-10,共6页China Feed
基 金:甘肃省重点研发计划项目(20YF3NA020);国家自然科学基金-地区基金项目(31860659);甘肃省科学院-应用开发计划项目(2020JK-03)。
摘 要:厌氧真菌能降解木质纤维素,一些甲烷菌可以增强厌氧真菌的降解能力。将犏牛瘤胃厌氧真菌和甲烷菌共培养物(N.frontalis+M.olleyae)Cattle-YakTZ1降解秸秆产生的发酵液预处理玉米秸秆48 h后进行黄贮,以市售木聚糖酶做对照组,空白组不进行预处理。结果表明,空白组在发酵45 d后感官品质极差,DM流失率较高,ADF和NDF降解效果不明显,pH值为6~7,已严重发生霉变;对照组与试验组在发酵过程中DM得到有效保存,NDF、ADF含量显著降低,试验组的乳酸含量显著高于对照组,丁酸检出量较低,CP含量显著高于空白组。(N.frontalis+M.olleyae)Cattle-YakTZ1发酵液预处理玉米秸秆后进行黄贮显著提升了黄贮感官品质、发酵特性营养成分,具有独特的应用价值。Anaerobic fungi can degrade lignocellulose,and some methanogens can enhance the degradation ability of anaerobic fungi.To improve the yellow storage quality of corn straw and increase its feeding value,this study pretreated corn stover with the fermentation liquid produced by Cattle-YakTZ1,a co-culture of anaerobic fungi and methanogen(N.frontalis+M.olleyae)from yak cattle for 48 hours,before ensiling,with commercial xylanase as the control group.The results showed that the blank group had poor sensory quality,high DM loss rate,insignificant ADF and NDF degradation effects,pH between 6~7,and serious mold growth after 45 days of fermentation.The control group and experimental group effectively preserved DM during fermentation,significantly reduced NDF and ADF content.The experimental group had significantly higher lactate content than the control group,lower butyrate detected,and significantly higher CP content than the blank group.The pretreatment of corn straw with(N.frontalis+M.olleyae)Cattle-YakTZ1 fermentation broth significantly improved the sensory quality,fermentation characteristics and nutritional composition of the yellow storage.
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