东北春播区糜子核心种质的荧光微卫星标记鉴定  

Core germplasm identification of proso millet in spring sowing area of Northeast China using fluorescent microsatellite markers

作　　者：丁艺冰 辛旭霞 冯智尊 郭娟 曹越 陈喜明[2] 王晓丹 曹晓宁[3] SANTRA Dipak K 陈凌 乔治军[3] 王瑞云 DING Yi-Bing;XIN Xu-Xia;FENG Zhi-Zun;GUO Juan;CAO Yue;CHEN Xi-Ming;WANGXiao-Dan;CAO Xiao-Ning;SANTRA Dipak K;CHEN Ling;QIAO Zhi-Jun;WANG Rui-Yun(Agronomy College,Shanxi Agricultural University,Taigu 030801,Shanxi,China;Corn Research Institute,Shanxi Agricultural University,Xinzhou 034000,Shanxi,China;Center for Agricultural Genetic Resources Research,Shanxi Agricultural University/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture and Rural Affairs/Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops,Taiyuan 030031,Shanxi,China;Panhandle Research&Extension Center,Department of Agronomy and Horticulture,University of Nebraska-Lincoln,Scottsbluff 69361,Nebraska,USA)

机构地区：[1]山西农业大学农学院,中国山西太谷030801 [2]山西农业大学玉米研究所,中国山西忻州034000 [3]山西农业大学农业基因资源研究中心/农业农村部黄土高原作物基因资源与种质创制重点实验室/杂粮种质资源发掘与遗传改良山西省重点实验室,中国山西太原030031 [4]内布拉斯加大学林肯分校农艺系小宗粮豆研究与推广中心,美国内布拉斯加州斯克茨布拉夫69361

出　　处：《作物学报》2024年第7期1728-1739,共12页Acta Agronomica Sinica

基　　金：山西农业大学杂粮种质创新与分子育种国家实验室(筹)项目(202204010910001);国家自然科学基金项目(31271791);财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-06-14.5-A16);山西省现代农业产业技术体系建设专项资金项目(2023CYJSTX03-12);山西省重点研发项目(2022ZDYF110);山西农业大学杂粮种质创新与分子育种山西省重点实验室项目(K462202040);山西农业大学生物育种工程项目(YZGC069);山西农业大学农学院研究生教育改革项目(2023YJG05)资助。

摘　　要：优异种质资源是糜子新品种选育和产业发展的基础。本研究以190份东北春播区糜子核心种质为材料,利用前期构建的SSR标记在5'端标注荧光,进行PCR扩增和毛细管电泳。根据毛细管电泳检测的片段有无采用“0/1”表示,利用ID Analysis4.0进行区分,使用PopGene、PowerMarker、MEGA、Structure、NTSYS进行遗传多样性分析。试验结果表明,3个荧光SSR标记组合(RYW3+RYW6+RYW28)可区分190份材料,共检测出等位变异73个,平均为24.3333;有效等位基因数(Ne)为5.4728(RYW3)~15.8922(RYW6),平均为9.6496;检出Shannon多样性指数(I)为2.0851(RYW3)~2.9457(RYW6),平均为2.4896;观测杂合度(Ho)为0.7529(RYW6)~0.9574(RYW28),平均为0.8876;期望观测杂合度(He)为0.8194(RYW3)~0.9398(RYW6),平均为0.8765;Nei’s基因多样性指数(Nei)为0.8173(RYW3)~0.9371(RYW6),平均为0.8741;多态性信息含量(PIC)为0.8656(RYW3)~0.9722(RYW6),平均为0.9198。基于UPGMA将190份资源划分为3个群组。基于Structure的遗传结构分析(K=3)将糜子核心种质分为3个类群,来源于东北地区的4个基因库(黑龙江、吉林、辽宁和内蒙古的一部分)。基于主成分分析将材料分为4个类群,与地理来源一致。利用在线二维码技术(https://cli.im/)构建了190份东北糜子核心种质的DNA分子身份证。Rich germplasm resources are the basis of new varieties breeding and industrial development in proso millet.In this study,190 core germplasms of proso millet in spring sowing area of northeast China were used as the experimental materials.PCR amplification and capillary electrophoresis were performed using the previously constructed SSR markers labeled with fluorescence at the 5'end.According to the fragments detected by capillary electrophoresis,“0/1”was used to represent without of the fragments,and ID Analysis 4.0 was used to distinguish them.PopGene,PowerMarker,MEGA,Structure,and NTSYS were used for genetic diversity analysis.The results showed that 190 materials could be distinguished by three fluorescent SSR(RYW3,RYW6,RYW28)marker combinations.A total of 73 alleles were detected,with an average of 24.3333.The effective number of alleles(Ne)was 5.4728(RYW3)–15.8922(RYW6),with an average of 9.6469.The Shannon diversity index(I)was 2.0851(RYW3)–2.9457(RYW6),with an average of 2.4896.The observed heterozygosity(Ho)was 0.7529(RYW6)–0.9574(RYW28),with an average of 0.8876.The expected observed heterozygosity(He)was 0.8194(RYW3)–0.9398(RYW6),with an average of 0.8765.The Nei’s gene diversity index(Nei)was 0.8173(RYW3)–0.9371(RYW6),with an average of 0.8741.The polymorphism information content(PIC)was 0.8656(RYW3)–0.9722(RYW6),with an average of 0.9198.Based on UPGMA,190 resources were divided into three groups.Based on the genetic structure of Structure(K=3),the core collection of proso millet was divided into three groups.The proso millet was derived from four provinces in northeast China(Heilongjiang,Jilin,Liaoning,and the part of Inner Mongolia).Based on principal component analysis,the materials were divided into four groups,which was consistent with their geographical origin.The DNA molecular identification cards of 190 core collections of northeast proso millet were constructed using online two-dimensional code technology(https://cli.im/).

关 键 词：糜子 东北春播区 荧光微卫星标记 毛细管电泳 DNA分子身份证 

分 类 号：S516[农业科学—作物学]