绿樟肖叶甲肠道细菌多样性分析及功能菌株的筛选  

Analysis of Intestinal Bacterial Diversity and Screening of Functional Strains of Chalcolema cribrata Chen from Camphora longepaniculata(Gamble)Y.Yang,Bing Liu&Zhi Yang

作　　者：胡连清 彭俊琪 陈露 刘雯雯 冯瑞章[1,2] 周万海 魏琴 秦晓红[1] 查黎萍 HU Lianqing;PENG Junqi;CHEN Lu;LIU Wenwen;FENG Ruizhang;ZHOU Wanhai;WEI Qin;QING Xiao-hong;ZHA Liping(Faculty of Agriculture,Forestry and Food Engineering,Yibin University,Yibin,Sichuan 644000,China;Key Lab of Aromatic Plant Re-sources Exploitation and Utilization in Sichuan Higher Education,Yibin,Sichuan 644000,China)

机构地区：[1]宜宾学院农林与食品工程学部,四川宜宾644000 [2]香料植物资源开发与利用四川省高校重点实验室,四川宜宾644000

出　　处：《宜宾学院学报》2024年第6期102-108,共7页Journal of Yibin University

基　　金：四川省自然科学基金项目(2022NSFSC0176);宜宾学院培育项目(2021PY68);四川省油樟工程技术研究中心开放基金项目(2021YZY06)。

摘　　要：为分析绿樟肖叶甲肠道内生细菌群落结构及功能特点,采用高通量测序技术与纯培养技术相结合的方法分析绿樟肖叶甲肠道内生细菌多样性及群落结构,并通过选择培养基筛选其中的功能菌. Illumina MiSeq测序结果表明,绿樟肖叶甲肠道内生细菌隶属于15门、24纲、56目、97科、140属;通过COG功能分类统计发现,绿樟肖叶甲肠道中参与“碳水化合物运输和代谢”及“氨基酸转运与代谢”功能的菌群的相对丰度较高.纯培养结果显示,从绿樟肖叶甲肠道分离的37株内生细菌隶属于4门4纲4目4科9属;通过功能选择培养基筛选出23株产蛋白酶功能菌、6株产酯酶功能菌、3株产脂肪酶功能菌.这表明绿樟肖叶甲肠道内生细菌的群落多样且功能丰富.To analyze the community structure and functional characteristics of endophytic bacteria of Chalcolema cribrata Chen,the diversity and community structure of endophytic bacteria in Chalcolema cribrata Chen were investigated and analyzed using high-throughput sequencing and pure culture,and the functional bacteria were screened through selection of culture medium.The results of Illumina MiSeq sequencing show that intestinal bacterials of the Chalcolema cribrata Chen belong to 15 phyla,24 classes,56 orders,97 families and 140 genera;through Clusters of Orthologous Groups functional classification statistics,it is found that the relative abundance of bacterial communities involved in“carbohydrate transport and metabolism”and“amino acid transport and metabolism”functions in the intestine of Chalcolema cribrata Chen is relatively high.The results of pure culture show that 37 strains of bacteria isolated from the intestinal tract of Chalcolema cribrata Chen belong to 4 phyla,4 classes,4 orders,4 fami⁃lies and 9 genera.23 protease producing strains,6 esterase producing strains and 3 lipase producing strains aere screened by func⁃tional selection medium.This indicates that intestine of Chalcolema cribrata Chen is diverse and functional.

关 键 词：绿樟肖叶甲 肠道细菌 多样性分析 高通量测序技术 纯培养 

分 类 号：Q93-33[生物学—微生物学]