葛根素对小鼠非酒精性脂肪肝保护作用的研究  

Protection of Nonalcoholic Fatty Liver in Mice by Puerarin

作　　者：张桐萃 姚若瑾 王超 吴佳乐 李函擎 王申涛 ZHANG Tongcui;YAO Ruojin;WANG Chao;WU Jiale;LI Hanqing;WANG Shentao(College of Clinical Medicine,Qilu Medical University,Shandong Zibo 255213,China;Department of Pathology,Qilu Medical University,Shandong Zibo 255300,China)

机构地区：[1]齐鲁医药学院临床医学院,山东淄博255213 [2]齐鲁医药学院病理学教研室,山东淄博255300

出　　处：《生物技术进展》2024年第3期480-485,共6页Current Biotechnology

基　　金：山东省教学研究项目(2023JX037);齐鲁医药学院教学改革研究项目(XJJY2147)。

摘　　要：目前,非酒精性脂肪肝病已成为肝脏相关疾病的重要研究对象,我国非酒精性脂肪肝患病率也呈逐年上升趋势。然而,非酒精性脂肪肝的治疗方案比较局限,其中中药在其治疗中发挥了优势。将40只健康雄性小鼠随机分成5组:正常组、模型组、低剂量组、中剂量组、高剂量组,每组8只。除正常组外,其他组按照改良的造模方法高脂喂食35 d制备非酒精性脂肪肝模型(nonalcoholic fatty liver disease,NAFLD)。从第36天起低剂量组、中剂量组和高剂量组(60、80、100 mg·kg-1)将葛根素溶解在生理盐水中进行灌胃,其余组小鼠按照灌胃体积公式灌胃生理盐水。给药16 d后对小鼠血清中甘油三酯(triglyceride,TG)、胆固醇(total cholesterol,TC)、高密度脂蛋白(high-density lipoprotein,HDL)、低密度脂蛋白(low-density lipoproteins,LDL)指标进行检测,并取出小鼠的肝脏制备病理切片染色。研究结果显示,中、高剂量的葛根素对NAFLD模型小鼠的血脂相关指标(TC、TG、LDL)有较显著的降低作用,且高剂量的葛根素降低TG的效果最明显(P<0.01)。研究结果表明葛根素对小鼠非酒精性脂肪肝可能有一定的保护作用。Nowadays,non-alcoholic fatty liver disease(NAFLD) has become an important research object of liver-related diseases,and the prevalence of NAFLD in China has been increasing year by year.However,the treatment options for NAFLD are still relatively limited,and traditional Chinese medicine has played an advantageous role in its treatment.In this experiment,forty healthy male mice were randomly divided into five groups of eight mice each:normal group,model group,low-dose group,medium-dose group,and high-dose group.The model of NAFLD was prepared by high-fat feeding for 35 days according to the improved modeling method,except for the normal group.From day 36 onwards,the low-dose,medium-dose and high-dose groups(60,80,100 mg·kg-1) were gavaged with geraniol dissolved in saline,and the rest of the groups were gavaged with saline according to the formula of gavage volume.After 16 days of administration,triglyceride(TG),total cholesterol(TC),high-density lipoprotein(HDL),low-density lipoprotein(LDL) indexes were tested,and the livers of the mice were removed to prepare pathological sections.The experimental results showed that the middle and high doses of puerarin had a more significant lowering effect on the lipid-related indexes(TC,TG,LDL) of the NAFLD model mice,and the effect of the high dose of puerarin in lowering TG was more obvious(P<0.01).The experimental results suggested that puerarin may have a certain protective effect on NAFLD in mice.

关 键 词：葛根素 非酒精性脂肪肝 小鼠 保护作用 肝细胞脂肪变性 

分 类 号：R285.5[医药卫生—中药学]