接种氧化亚氮(N_(2)O)还原细菌YSQ030对复垦土壤N_(2)O排放和氮循环关键功能基因的影响  

Effects of inoculation with N2 O-reducing bacteria YSQ030 on soil N2 O emission and key functional genes involved in nitrogen cycling in reclaimed soil

作　　者：朱津宏 熊若男 杨思琪 高南 吴永红[3] 张振超[4] 吴国平[4] 申卫收 ZHU Jinhong;XIONG Ruonan;YANG Siqi;GAO Nan;WU Yonghong;ZHANG Zhenchao;WU Guoping;SHEN Weishou(School of Environmental Science and Engineering/Jiangsu Key Laboratory of Atmospheric Environment Monitoring&Pollution Control/Collaborative Innovation Center of Atmospheric Environment and Equipment Technology,Nanjing University of Information Science&Technology,Nanjing 210044,China;College of Biotechnology and Pharmaceutical Engineering/National Engineering Research Center for Biotechnology,Nanjing Tech University,Nanjing 211816,China;State Key Laboratory of Soil&Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008,China;Zhenjiang Institute of Agricultural Sciences in Hilly Area of Jiangsu Province,Zhenjiang 212400,China)

机构地区：[1]南京信息工程大学环境科学与工程学院/江苏省大气环境监测与污染控制高技术研究重点实验室/大气环境与装备技术协同创新中心,南京210044 [2]南京工业大学生物与制药工程学院/国家生化工程技术研究中心,南京211816 [3]中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,南京210008 [4]江苏丘陵地区镇江农业科学研究所,镇江212400

出　　处：《南京信息工程大学学报》2024年第3期416-427,共12页Journal of Nanjing University of Information Science & Technology（Natural Science Edition）

基　　金：国家自然科学基金(31972503,41771291);江苏省重点研发计划(社会发展)项目(BE2020731)。

摘　　要：复垦土地是重要的后备土地资源,但通常土壤结构差、有机质和养分含量低;增施有机肥是快速提升地力的关键途径,但会造成温室气体如氧化亚氮(N_(2)O)的大量排放.接种具有N_(2)O还原功能的植物根际促生菌(PGPR)不仅能够减少温室气体排放,还能促进作物生长.本研究以一株具有N_(2)O还原功能的PGPR反硝化无色杆菌(Achromobacter denitrificans)YSQ030为供试菌株,明确接种YSQ030对施用有机肥的复垦土壤N_(2)O排放和氮循环关键功能基因的影响.通过设置施用有机无机复混肥和羊粪有机肥的土壤微宇宙试验,利用气相色谱仪分析接种YSQ030后土壤N_(2)O排放通量,进一步计算累积排放量;在试验结束后分析土壤pH、EC(电导率)、硝态氮和铵态氮含量,并利用实时荧光定量PRC分析土壤硝化功能基因(AOA amoA和AOB amoA)和反硝化功能基因(nirS、nirK、nosZⅠ和nosZⅡ)的丰度.结果显示,施用有机无机复混肥和羊粪有机肥的土壤中接种YSQ030明显减少复垦土壤N_(2)O排放,N_(2)O排放量最大减少分别达90.4%和30.6%.施用有机无机复混肥处理的N_(2)O排放量远高于施用羊粪有机肥处理,这可能是由于施用有机无机复混肥的土壤与施用羊粪有机肥的土壤相比,土壤中编码反硝化细菌N_(2)O还原酶基因nosZⅠ和非典型反硝化细菌N_(2)O还原酶基因nosZⅡ基因丰度较低.施用有机无机复混肥均显著降低土壤硝化和反硝化功能基因丰度,而施用羊粪有机肥对土壤硝化和反硝化功能基因丰度大多没有明显影响.本研究表明,接种YSQ030能够减少施用有机肥土壤的N_(2)O排放,将为复垦土壤地力提升和N_(2)O减排提供科学依据,也将为研发新型微生物肥料或生物有机肥提供核心菌种资源.Though the reclaimed land is an important reserve land resource,it usually is poor in soil structure and low in organic matter and nutrient content.Organic fertilizer can quickly improve soil productivity,yet it will cause large emissions of greenhouse gases such as Nitrous Oxide(N2O).It has been proved that the inoculation of Plant Growth-Promoting Rhizobacteria(PGPR)with N2O reduction function not only reduces greenhouse gas emissions but also promotes crop growth.In this study,a PGPR denitrificans YSQ030 with N2O reduction function was used as the test strain to clarify the effect of YSQ030 on N2O emission and nitrogen cycling key functional genes in re-claimed soil with organic fertilizer application.Soil microcosmic experiments were set up for application of organic-inorganic compound fertilizer and sheep manure,then the soil N2O emission fluxes after inoculation of YSQ030 were analyzed by gas chromatography.Meanwhile,soil chemical properties were analyzed at the end of the experiment,and the abundance of soil nitrification and denitrification functional genes(AOA amoA and AOB amoA;nirS,nirK,nosZⅠand nosZⅡ)were analyzed by real-time quantitative PRC.The results showed that YSQ030 significantly re-duced the N2O emission of reclaimed soil with organic-inorganic compound fertilizer or sheep manure,with the max-imum reduction of N2O emission reaching 915%and 309%,respectively.The N2O emissions of organic-inorganic compound fertilizer treatment were much higher than those of sheep manure treatment,which may be due to the low abundance of N2O reductase genes of nosZⅠand nosZⅡin the former treatment.Furthermore,significant reduc-tion of the abundance of nitrification and denitrification functional genes were observed only in organic-inorganic compound fertilizer treatments.This study shows that YSQ030 can reduce the N2O emission in soil applied with or-ganic fertilizer,which can provide a scientific basis for both soil fertility improvement and N2O emission reduction,and also provide core strain resources f

关 键 词：微生物肥料 有机肥 氧化亚氮(N_(2)O) 植物根际促生菌 温室气体减排 

分 类 号：S144[农业科学—肥料学]