抗痛蝎多肽的分离与筛选  

Isolation and screening of anti-pain scorpion peptides

作　　者：王玉 张晓婷 姜鸣 吴艳艳 杨亮 杨清湖[1] 刘霞[1] 白占涛[1] WANG Yu;ZHANG Xiaoting;JIANG Ming;WU Yanyan;YANG Liang;YANG Qinghu;LIU Xia;BAI Zhantao(School of Life Sciences,Shaanxi Provincial Engineering Research Center for Conservation and Utilization of Biological Resources,Yan’an University,Yan’an 716000,China)

机构地区：[1]延安大学生命科学学院,陕西省区域生物资源保育与利用工程技术研究中心,陕西延安716000

出　　处：《延安大学学报（自然科学版）》2024年第2期37-42,48,共7页Journal of Yan'an University：Natural Science Edition

基　　金：陕西省秦创原“科学家+工程师”队伍建设项目(2022KXJ-152);陕西省重点研发计划项目(2023-YBSF-543,2024NC-YBXM-172);陕西省教育厅服务地方专项计划项目(22JC060);延安大学科研计划项目(2023TBZR-004,2023KXJ-001)。

摘　　要：东亚钳蝎(Buthusmartensii Karsch)是我国重要的中药材之一,其毒液富含多种离子通道及受体的调制分子,对疼痛,癫痫等疾病有治疗作用。因此,针对蝎毒素中复杂多肽的分离、筛选与鉴定是蝎毒素研究与开发的重点。为了分离并筛选出具有镇痛效果的蝎毒多肽,实验利用反相高效液相色谱的方法分离纯化蝎毒,将得到的4种蝎毒多肽组分分别命名为BmKⅡ-10、BmKⅡ-11、BmKⅡ-16和BmKⅡ-24,结合质谱技术鉴定其分子量分别为7530.8 Da、7366.0 Da、5949.6 Da和6852.4 Da。通过构建疼痛模型检测蝎毒多肽组分对小鼠疼痛的影响,结果显示,BmKⅡ-10,BmKⅡ-11可能为蝎毒中的致痛组分,而BmKⅡ-16具有显著的镇痛效果,能有效抑制由福尔马林和角叉菜胶引起的自发痛与机械痛行为。本研究所采用的多肽分离纯化方法能有效获得蝎毒素中多种多肽组分,进一步验证发现部分组分与疼痛密切相关,为疼痛药物的开发与应用提供了理论和实验依据。The Buthusmartensii Karsch is one of the important Chinese medicinal materials in China.Its venom is rich in modulating molecules of various ion channels and receptors,which has therapeutic effects on pain,epilepsy and other diseases.Therefore,the isolation,screening and identification of complex peptides in scorpion toxin have been the focus of research and development of scorpion toxins.In order to isolate and screen the scorpion venom polypeptide with analgesic effects,four scorpion venom peptide components were separated and purified by reversed-phase high performance liquid chromatography,named BmKⅡ-10,BmKⅡ-11,BmKⅡ-16 and BmKⅡ-24,and their molecular weights were 7530.8 Da,7366.0 Da,5949.6 Da and 6852.4 Da,respectively.A pain model was constructed to examin the effects of scorpion venom polypeptide components on pain in mice.The results showed that BmKⅡ-10 and BmKⅡ-11 may be the pain-causing components in scorpion venom,while BmKⅡ-16 had a significant analgesic effect and could effectively inhibit spontaneous pain and mechanical pain caused by formalin and carrageenan.The peptide separation and purification method used in this study can effectively obtain a variety of peptide components in scorpion toxin,and further verify that some components are closely related to pain,which provides theoretical and experimental basis for the development and application of pain drugs.

关 键 词：东亚钳蝎 蝎毒多肽 分离纯化 抗痛 

分 类 号：Q74[生物学—分子生物学]