静电纺PLGA-g-PVP/I_(n)^(-)多孔抗菌纤维膜的制备及其性能研究  

Preparation and properties of electrospun PLGA-g-PVP/I_(n)^(-) porous antibacterial fiber membrane

作　　者：先丽 倪茂君[1] 张晓彬 胡椹 王静霞[1] 彭朝荣 Xian Li;Ni Maojun;Zhang Xiaobin;Hu Zhen;Wang Jingxia;Peng Chaorong(Sichuan Institute of Atomic Energy,Chengdu 610101;Irradiation Preservation Key Laboratory of Sichuan Province,Chengdu 610101)

机构地区：[1]四川省原子能研究院,成都610101 [2]辐照保藏四川省重点实验室,成都610101

出　　处：《化工新型材料》2024年第6期259-263,共5页New Chemical Materials

基　　金：四川省科技创新人才项目(2021JDRC0018);四川省科技支撑项目(2021YFS0092,2022YFSY0003,2020YJ0222,2021YJ0326,23QYCX0026);2023年四川省省级科研院所基本科研业务。

摘　　要：抗菌复合膜是修复皮肤损伤和预防皮肤感染的重要医用材料。采用^(60)Co-γ射线辐照接枝聚合法将N-乙烯基吡咯烷酮(NVP)单体接枝到聚乳酸-羟基乙酸共聚物(PLGA)上,形成聚乳酸-羟基乙酸共聚物接枝聚乙烯基吡咯烷酮(PLGA-g-PVP)链,通过碘和聚乙烯基吡咯烷酮(PVP)的络合反应,将聚碘化物固定在PLGA表面,得到聚乳酸-羟基乙酸共聚物接枝聚乙烯基吡咯烷酮/聚碘化物(PLGA-g-PVP/I_(n)^(-))复合物。将复合物溶于二氯甲烷和N,N-二甲基甲酰胺(DMF)溶液中,采用静电纺丝技术制备了多孔抗菌纤维膜。通过凝胶渗透色谱仪、电感耦合等离子体质谱仪、傅里叶变换红外光谱仪、拉曼光谱仪、全自动物理吸附仪和扫描电子显微镜等分析手段对多孔抗菌纤维膜进行微观结构和成分分析,用金黄色葡萄球菌和大肠杆菌评价其抗菌活性。结果表明,金黄色葡萄球菌和大肠杆菌浓度为2.1×10^(4)CFU/mL和4×10^(4)CFU/mL时,该材料具有良好的抑菌效果,且对L929细胞有良好的生物相容性,细胞毒性为0级,可以作为恢复皮肤损伤和防止感染的医用材料。Antibacterial composite membrane is an important medical material for skin injury restoration and infection prevention.Polymerization.PLGA-g-PVP(poly(lactic acid)-hydroxyacetic acid copolymer grafted with poly(vinylpyrrolidone))chains were formed ^(60)Co-γ-irradiation grafting polymerization of N-vinylpyrrolidone(NVP)monomer grafted onto PLGA.Polyiodide was fixed on the surface of PLGA through the coordination reaction of iodine and PVP to obtain PLGA-g-PVP/In-composite.The modified composite was dissolved in methylene chloride and DMF solution,and a porous antibacterial fiber membrane was prepared using electrospinning technology.The microstructure and composition of PLGA-g-PVP/I_(n)^(-) membrane were analyzed by GPC,ICP-MS,FT-IR,Raman,BET and SEM method.The antibacterial activity of PLGA-g-PVP/I-n membrane was evaluated by Staphylococcus aureus and Escherichia coli.The results showed that the composite had good antibacterial activity at the concentration of 2.1×10^(4) CFU/mL and 4×10^(4)CFU/mL,and had good biocompatibility with L929 cells,with a cytotoxicity rating of 0,making it a potential medical material for skin injury restoration and infection prevention.

关 键 词：辐照接枝 静电纺丝 高聚碘 抗菌 

分 类 号：TQ340.64[化学工程—化纤工业] TB34[一般工业技术—材料科学与工程]