地衣芽孢杆菌HR高密度培养及代谢产物解析  

High-density culture and metabolite analysis of Bacillus licheniformis HR

作　　者：侯瑞 杨灿 郑佳 曾山 陈燕梅 王川 刘超兰[3] 郭义东[3] HOU Rui;YANG Can;ZHENG Jia;ZENG Shan;CHEN Yanmei;WANG Chuan;LIU Chaolan;GUO Yidong(Sichuan University of Science&Engineering,Yibin,Sichuan Province 644000,China;Wuliangye Yibin Co.,ltd,Yibin,Sichuan Province 644000,China;China Novel Antibiotics Cultural Collection Center,School of Pharmacy,Chengdu University,Chengdu,Sichuan Province 610106,China)

机构地区：[1]四川轻化工大学,四川宜宾644000 [2]宜宾五粮液股份有限公司,四川宜宾644000 [3]国家新抗生素菌种保藏管理中心,成都大学,四川成都610106

出　　处：《中国饲料》2024年第11期53-60,共8页China Feed

基　　金：成都大学国家新抗生素菌种保藏管理中心开放基金项目(CCSIIA202203,CCSIIA202201);四川省科技计划重点研发项目(2023YFN0020)。

摘　　要：本试验旨在对地衣芽孢杆菌培养基进行优化,确定其最佳培养条件,为工业化生产是提供依据。以活菌数和OD_(650nm)为检测指标,对培养基和培养条件进行优化,并扩大至50 L罐规模。利用GC-MS检测分析培养液中挥发性物质并解析。结果表明:地衣芽孢杆菌培养的最佳条件为α-乳糖2 g/L、豆粕20 g/L、MnSO_(4)1 g/L、FeSO_(4)0.01 g/L、MgSO_(4)0.04 g/L、K_(2)HPO_(4)2 g/L,接种量4%,培养时间为24 h,初始pH为7,在50 L发酵罐培养其活菌数最大值为1.36×10^(9)CFU/mL。GC-MS共检测出12种物质,其中3,5-二甲基-1,3,4-己三醇、L-乳酸异丁酸和2-甲基丁酸对动物机体都有不同程度的益生效果。使用优化后的条件进行地衣芽孢杆菌HR培养,培养液中活菌数有所提高,从而提高了其生产效率,对其产业化推进有一定现实意义。The aim of this experiment was to optimize the culture medium of Bacillus licheniformis and determine its optimal culture conditions,providing the basis for industrialization.With the number of viable bacteria and OD_(650nm)as the detection indexes,the medium and culture conditions were optimized and expanded to the size of 50 L tank.GC-MS was used to detect and analyze the volatile substances in the culture medium.The results showed that the optimum conditions for Bacillus licheniformis culture wereα-lactose 2 g/L,soybean meal 20 g/L,MnSO_(4)1 g/L,FeSO_(4)0.01 g/L,MgSO_(4)0.04 g/L,K_(2)HPO_(4)2 g/L,4%inoculation amount,24 h incubation time,initial pH 7.The maximum number of viable bacteria cultured in 50 L fermenter was 1.36×10^(9)CFU/mL.A total of 12 substances were detected by GC-MS,among which 3,5-dimethyl-1,3,4-hexyl alcohol,L-lactic isobutyric acid and 2-methylbutyric acid had different degrees of prebiotic effects on animal body.The optimized conditions were used for HR culture of Bacillus licheniformis,and the number of viable bacteria in the culture medium was increased,thus improving its production efficiency and having certain practical significance for its industrialization.

关 键 词：地衣芽孢杆菌 培养条件 优化 培养基 

分 类 号：S816.3[农业科学—饲料科学]