低温下苜蓿生理代谢及抗寒基因差异分析  

Analysis of differences in physiological metabolism and cold resistance genes of alfalfa under low temperature

作　　者：王晓龙 杨曌 柴华 李莎莎 徐艳霞 吴玥 高海娟 王建丽[2] 王锐 李伟 王若丁 张杨 WANG Xiaolong;YANG Zhao;CHAI Hua;LI Shasha;XU Yanxia;WU Yue;GAO Haijuan;WANG Jianli;WANG Rui;LI Wei;WANG Ruoding;ZHANG Yang(Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences/Heilongjiang Provincial Engineering Technology Research Centreof Forage Breeding and Germplasm Resource Utilization,Qiqihar,Heilongjiang Province 161005,China;Institute of Grass Science of Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang Province 150086,China)

机构地区：[1]黑龙江省农业科学院畜牧兽医分院/黑龙江省牧草育种与种质资源利用工程技术研究中心,黑龙江齐齐哈尔161005 [2]黑龙江省农业科学院草业研究所,黑龙江哈尔滨150086

出　　处：《中国饲料》2024年第11期180-186,共7页China Feed

基　　金：黑龙江省自然科学基金资助项目(YQ2022C033);科技创新2030—重大项目(2022ZD04012);齐齐哈尔市科技计划项目(CNYGG-2023009)。

摘　　要：本试验旨在筛选适宜黑龙江西部地区种植的耐寒苜蓿种质材料,探究低温胁迫下苜蓿幼苗的生理响应机制,为苜蓿耐寒种质资源鉴定提供理论依据。以4个苜蓿品种为试验材料,通过低温处理测定生理指标游离脯氨酸(Pro)、可溶性糖(SS)、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、相对电导率和基因NAC3、CAS18表达量,利用隶属函数评价筛选出耐寒苜蓿品种。结果表明:随低温胁迫程度加剧,供试苜蓿品种的Pro、SS、MDA和电导率呈现升高趋势,SOD和POD活性呈现先升后降的变化;重度胁迫时,肇东苜蓿的Pro、SS、SOD和POD均为最高,而电导率为最低;同时肇东苜蓿的NAC3、CAS18基因表达量显著高于其他品种(P<0.05)。运用隶属函数对品种的抗寒性进行了评价,得出苜蓿品种的抗寒强弱顺序为:肇东>皇后>420>赛迪。This study was aimed to explore the physiological response mechanism of alfalfa(Medicago sativa)seedlings under low temperature stress by screening cold-resistant alfalfa germplasm materials suitable for planting in western Heilongjiang,so as to provide theoretical basis for identification of cold-resistant alfalfa germplasm resources.Four alfalfa varieties were treated at low temperature.Physiological indexes proline(Pro),soluble sugar(SS),malondialdehyde(MDA),superoxide dismutase(SOD),peroxidase(POD),relative electrical conductivity and the expresion level of genes(NAC3、CAS18)were measured.The cold-resistant alfalfa varieties were screened by membership function evaluation.Results showed that with the aggravation of low temperature stress,the Pro,SS,MDA and relative electrical conductivity of tested alfalfa varieties increased,while SOD and POD activity increased first and then decreased.Under severe stress,the Pro,SS,SOD and POD of Zhaodong alfalfa were the highest,while the electrical conductivity was the lowest;Meanwhile,the gene expressions of NAC3 and CAS18 of Zhaodong alfalfa were significantly higher than those of other varieties(P<0.05).Membership function was used to evaluate the cold resistance of alfalfa varieties.The cold resistance of alfalfa varieties from high to low was as follows:Zhaodong>Queen>420>Sardi.

关 键 词：紫花苜蓿 幼苗期 低温 生理特性 抗寒基因 评价 

分 类 号：S816.5[农业科学—饲料科学]