人脐带间充质干细胞条件培养基及外泌体对肝癌细胞增殖、迁移、侵袭和凋亡的影响  

Effects of conditioned medium and exosomes of human umbilical cord mesenchymal stem cells on proliferation,migration,invasion,and apoptosis of hepatocellular carcinoma cells

作　　者：金凯 唐婷 李美乐 谢裕安 Jin Kai;Tang Ting;Li Meile;Xie Yuan(Graduate School,Guangxi University of Chinese Medicine,Nanning 530200,Guangxi Zhuang Autonomous Region,China;Guangxi Zhuang Autonomous Region Maternal and Child Healthcare Hospital,Nanning 530002,Guangxi Zhuang Autonomous Region,China;Guangxi Key Laboratory of Birth Defects and Stem Cell Biosample Bank,Nanning 530002,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]广西中医药大学研究生院,广西壮族自治区南宁市530002 [2]广西壮族自治区妇幼保健院,广西壮族自治区南宁市530002 [3]广西出生缺陷及干细胞生物样本库重点实验室,广西壮族自治区南宁市530002

出　　处：《中国组织工程研究》2025年第7期1350-1355,共6页Chinese Journal of Tissue Engineering Research

基　　金：广西出生缺陷及干细胞生物样本库重点实验室(ZTJ2020002),项目负责人:谢裕安;广西重点实验室运行补助项目(21-220-22),项目负责人:谢裕安。

摘　　要：背景:间充质干细胞可通过分泌含细胞因子、生长因子和外泌体的细胞外囊泡调节肿瘤微环境,对肿瘤细胞生物学行为进行精准调控。目的:研究人脐带间充质干细胞条件培养基及外泌体对肝癌细胞生物学特性的影响。方法:收集人脐带间充质干细胞培养上清,通过高速离心并过滤获得人脐带间充质干细胞条件培养基;收集人脐带间充质干细胞培养上清,通过超高速梯度离心法提取人脐带间充质干细胞外泌体。使用PKH26标记人脐带间充质干细胞外泌体,与肝癌细胞MHCC97-H共培养,荧光显微镜观察MHCC97-H细胞摄取外泌体情况。通过CCK-8增殖实验、Transwell迁移和侵袭实验以及流式凋亡实验评估人脐带间充质干细胞条件培养基、人脐带间充质干细胞外泌体对肝癌细胞生物学功能的影响。结果与结论:(1)人脐带间充质干细胞外泌体可被MHCC97-H细胞摄取且主要分布于细胞质中;(2)人脐带间充质干细胞条件培养基处理后,MHCC97-H细胞的增殖、迁移和侵袭能力显著增强(P<0.001,P<0.05,P<0.01),凋亡能力显著下降(P<0.001);而人脐带间充质干细胞外泌体处理后,MHCC97-H细胞的增殖能力下降(P<0.001),迁移、侵袭及凋亡能力显著增强(P<0.001);(3)结果表明,人脐带间充质干细胞条件培养基具有促进MHCC97-H细胞增殖、迁移、侵袭和抑制凋亡的能力;而人脐带间充质干细胞外泌体则具有促进MHCC97-H细胞迁移、侵袭及凋亡和抑制增殖的能力。BACKGROUND:Mesenchymal stem cells can regulate the tumor microenvironment by secreting extracellular vesicles containing cytokines,growth factors and exosomes for the precise regulation of biological behavior of tumor cells.OBJECTIVE:To investigate the effects of human umbilical cord-derived mesenchymal stem cell conditioned medium and their released exosomes on the biological properties of hepatocellular carcinoma cells.METHODS:Human umbilical cord mesenchymal stem cell supernatant was collected,centrifuged and filtered at high speed to obtain human umbilical cord mesenchymal stem cell conditioned medium.Human umbilical cord mesenchymal stem cell supernatant was collected and human umbilical cord mesenchymal stem cell exosomes were extracted by ultra-high speed gradient centrifugation.Human umbilical cord mesenchymal stem cell exosomes were labeled with PKH26 and co-cultured with hepatocellular carcinoma cell MHCC97-H.The uptake of exosomes by MHCC97-H cells was observed by fluorescence microscopy.The effects of human umbilical cord mesenchymal stem cell conditioned medium and human umbilical cord mesenchymal stem cell exosomes on biological functions of hepatocellular carcinoma cells were assessed by the CCK-8 proliferation assay,Transwell migration and invasion assay,and the apoptosis assay.RESULTS AND CONCLUSION:(1)Human umbilical cord mesenchymal stem cell exosomes could be uptaken by MHCC97-H cells and was mainly distributed in the cytoplasm.(2)After treatment with human umbilical cord mesenchymal stem cell conditioned medium,MHCC97-H cells showed a significant increase in proliferation,migration,and invasion(P<0.001,P<0.05,P<0.01),and a significant decrease in apoptosis(P<0.001),while after treatment with human umbilical cord mesenchymal stem cell exosomes,MHCC97-H cells showed a decrease in proliferation(P<0.001)and migration,invasion,and apoptosis were significantly enhanced(P<0.001).(3)The results indicated that human umbilical cord mesenchymal stem cell conditioned medium had the ability to promote the

关 键 词：肝癌 人脐带间充质干细胞 外泌体 条件培养基 增殖 迁移 侵袭 凋亡 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R735.7