不同倍性西瓜发育过程中瓜氨酸合成途径主要氨基酸含量变化及相关基因表达分析  

Changes of main amino acid contents in citrulline biosynthesis pathway and expression analysis of related genes during fruit development of watermelon with different ploidy

作　　者：陈子昊 路绪强[1] 何楠[1] 杨东东 朱红菊[1] 刘文革[1] CHEN Zihao;LU Xuqiang;HE Nan;YANG Dongdong;ZHU Hongju;LIU Wenge(Henan Joint International Research Laboratory of South Asian Fruits and Cucurbits/Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,Henan,China)

机构地区：[1]河南省南亚瓜果学国际联合实验室·中国农业科学院郑州果树研究所,郑州450009

出　　处：《中国瓜菜》2024年第6期18-26,共9页China Cucurbits And Vegetables

基　　金：河南省科技攻关项目(242102110242);中国农业科学院科技创新工程专项(CAAS-ASTIP-2021-ZFRI);国家现代农业产业技术体系(CARS-25-03);河南省重大科技专项(221100110400);河南省农业良种联合攻关项目(2022010503)。

摘　　要：以同源二倍体、三倍体和四倍体西瓜伊选为试验材料,研究3种倍性西瓜果实发育过程中瓜氨酸合成途径中谷氨酸、鸟氨酸、瓜氨酸和精氨酸4种氨基酸的含量差异以及其合成途径中关键酶基因的表达差异。结果表明,多倍体西瓜果实中瓜氨酸与鸟氨酸含量均显著高于二倍体,而多倍体精氨酸含量低于二倍体。在西瓜果实发育32 d时,瓜氨酸与鸟氨酸含量表现为三倍体>四倍体>二倍体,而谷氨酸与精氨酸含量表现为二倍体>四倍体>三倍体。多倍体与二倍体在果实发育后期(24~32 d)NAGS、NAGK、GAT、OTC基因表达量差异显著,二倍体中ASS1、ASS3、ARG基因表达量在整个果实发育期始终高于多倍体。以上结果表明,不同倍性西瓜果实中瓜氨酸含量存在差异可能是瓜氨酸合成途径上下游基因的差异表达造成的。研究结果对揭示不同倍性西瓜中瓜氨酸含量差异机制具有重要意义。The content of four amino acids,glutamic acid,ornithine,citrulline and arginine,as well as the expression differences of key enzyme genes in the citrulline synthesis pathway of citrulline,were studied in the diploid,triploid and tetraploid Yixuan watermelon.The results showed that the content of citrulline and ornithine in polyploid watermelon fruits were significantly higher than those in diploid,while the content of arginine in polyploid was lower than that in diploid.At 32 days of fruit development,the content of citrulline and ornithine were triploid>tetraploid>diploid,while the content of glutamic acid and arginine were diploid>tetraploid>triploid.Correspondingly,there were significant differences in the expression of NAGS,NAGK,GAT,OTC genes between polyploid and diploid in the late stage of fruit development,and the expression of ASS1,ASS3 and ARG genes in diploid was always higher than that of polyploid throughout the developmental period.These results indicated that the differences in citrulline content in different ploidy watermelon fruits were caused by the stable differential expression of upstream and downstream genes of citrulline synthesis pathway.This study is of great significance to reveal the mechanism of citrulline content difference in different ploidy watermelon.

关 键 词：西瓜 倍性 瓜氨酸 基因表达 

分 类 号：S651[农业科学—果树学]