大白菜根肿病抗感品种间的侵染过程及生理生化差异分析  被引量:2

Infection process and physiological and biochemical difference between clubroot-resistant and-susceptible Chinese cabbage lines

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作  者:苏贺楠 秦六月 杨双娟[1] 魏小春[1] 赵艳艳[1] 王志勇[1] 张文静 张晓伟[1] 原玉香[1] SU Henan;QIN Liuyue;YANG Shuangjuan;WEI Xiaochun;ZHAO Yanyan;WANG Zhiyong;ZHANG Wenjing;ZHANG Xiaowei;YUAN Yuxiang(Institute of Vegetables,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China)

机构地区:[1]河南省农业科学院蔬菜研究所,郑州450002

出  处:《中国瓜菜》2024年第6期45-51,共7页China Cucurbits And Vegetables

基  金:科创中原青年人才托举工程(2023HYTP022);河南省农业科学院优秀青年基金(2024YQ12);河南省优势学科联合基金重点项目(232301420024);河南省重点科技攻关(232102111105);河南省农业科学院创新团队(2023TD06)。

摘  要:根肿病是由芸薹根肿菌专性侵染十字花科蔬菜作物引起的一种世界性土传病害,对大白菜等蔬菜造成了严重的经济损失。以抗根肿病大白菜DH系(DH40R)和感病大白菜DH系(DH199S)为试验材料,通过观察根肿菌侵染的差异、防御酶活性、MDA和可溶性蛋白含量的测定研究大白菜根肿病抗性机制。结果表明,DH40R和DH199S的根毛侵染发生在接种后第2天,随着侵染时间的延长,出现皮层侵染,但DH40R的皮层侵染在接菌第8天后被阻断,对根肿病具有完全的抗性,而DH199S在接菌8 d后,根部有肿瘤产生。在接菌后2、8和13 d,分别对抗、感材料接菌和未接菌根部的防御酶活性、MDA和可溶性蛋白含量进行测定,发现DH40R在这3个时间点接菌后SOD的活性均显著高于未接菌对照组,在第8天达到最高峰值,显著高于DH199S;DH40R接菌后的CAT活性在第8天达到最高峰值1572.68μmol·mL^(-1)·min^(-1),显著高于DH40R未接菌对照组和接菌及未接菌处理的DH199S;在接菌后8和13 d,抗根肿病大白菜DH系DH40R的MDA含量显著低于接菌后的感病DH系DH199S;接菌后DH40R的可溶性蛋白含量在接菌后8和13 d均显著高于DH199S。研究结果可为后期解析大白菜根肿病抗性相关机制的研究奠定基础。Clubroot is a worldwide soil-borne disease of the cruciferous plants caused by Plasmodiophora brassicae,leading to serious economic losses to cabbage and other Brassica crops.In order to clarify the clubroot resistance mechanism of Brassica rapa,this study was conducted to detect the differences of infection process,defense enzyme activity and the content of MDA and soluble protein between clubroot-resistant(DH40R)and clubroot-susceptible(DH199S)Chinese cabbage lines.The results showed that the primary(root hair infection)stage was at 2 day after inoculation(dai)in both clubroot-resistant(DH40R)and clubroot-susceptible(DH199S)Chinese cabbage lines.With the extension of the infection time,cortical infection was blocked and showed complete P.brassicae resistance in DH40R,while in DH199S,swollen root occurred in the root after 8 dai.The defense enzyme activity and the content of MDA and soluble protein were measured at 2,8 and 13 dai.The activity of SOD in DH40R after inoculation is significantly higher than the mock at the whole experimental stage,the highest peak was reached at 8 dai,which was significantly higher than that of DH199S.The CAT activity of DH40R after inoculation reached the highest peak 1572.68μmol·mL^(-1)·min^(-1) at 8 dai,which was significantly higher than the mock of DH40R and the inoculated and uninoculated DH199S.The MDA content of DH40R was significantly lower than that of DH199S at 8 and 13 dai.The soluble protein content in DH40R was higher than that of DH199S at 8 and 13 dai. Overall, these results can lay foundation for further exploration of resistance mechanism of B. rapaagainst P. brassicae infection.

关 键 词:大白菜 根肿病 侵染过程 生理生化指标 

分 类 号:S634.1[农业科学—蔬菜学]

 

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