基于微流控技术的心肌细胞培养与刺激芯片  

Cardiomyocyte Culture and Stimulation Chip Based on Microfluidic Technology

作　　者：李浩正 唐森林 孙川婷 周元明 崔建国 Li Haozheng;Tang Senlin;Sun Chuanting;Zhou Yuanming;Cui Jianguo(School of Pharmacy and Bioengineering,Chongqing University of Technology,Chongqing 400054,China)

机构地区：[1]重庆理工大学药学与生物工程学院,重庆400054

出　　处：《微纳电子技术》2024年第6期75-82,共8页Micronanoelectronic Technology

基　　金：重庆市技术创新与应用示范(社会民生类)一般项目(cstc2018jscx-msyb0290);重庆理工大学研究生教育高质量发展项目资助(CYS22675)。

摘　　要：传统细胞培养皿或瓶对于心肌细胞培养来说空间过于庞大,不能很好地模拟出细胞在体生长的环境。研制了一种能将心肌细胞培养与刺激相结合的微流控芯片,构建了可对细胞施加气动拉伸刺激和电刺激的联合刺激体系,并使用此刺激体系进行体外心肌细胞培养,细胞存活率均在95%以上。通过对气动拉伸刺激(气压为0.5、1、3 kPa)、电刺激(频率为1、2、4 Hz)以及力电联合刺激(3 kPa+4 Hz)下的心肌细胞进行Cx43蛋白染色,验证了各种刺激均能促进Cx43蛋白的表达,且在不同刺激参数下效果差异明显,证实了该芯片可作为一种平台在体外进行心肌细胞的培养与刺激。Traditional cell culture dishes or vials are too large for cardiomyocyte culture and can not well simulate the environment in which cells growing in vivo.A microfluidic chip that can combine cardiomyocyte culture with stimulation was developed,and a combined stimulation system that can apply pneumatic stretching stimulation and electrical stimulation to cells was constructed.The stimulation system was used to culture cardiomyocytes in vitro,and the survival rate of cells is above 95%.Cx43 protein staining was performed on cardiomyocytes subjected to pneumatic stretching stimulation(0.5,1,3 kPa of air pressure),electrical stimulation(1,2,4 Hz of frequency)and combined electro-mechanical stimulation(3 kPa+4 Hz).It is verified that all kinds of stimulation cab promote Cx43 protein expression,and the effects are significantly different under different stimulation parameters.It is proved that the chip can be used as a platform to culture and stimulate cardiomyocytes in vitro.

关 键 词：微流控芯片 气动拉伸刺激 心肌细胞培养 电刺激 心肌细胞成熟 

分 类 号：R318.11[医药卫生—生物医学工程] TN40[医药卫生—基础医学]