畜禽粪便源有机肥中四环素类抗生素及转化产物的分布特征  

Occurrence of tetracyclines and their transformation products in manure-based organic fertilizers

作　　者：吴丹妮 冯皓迪 代世婷 马春萌 杨敏[2,4] 张昱 WU Danni;FENG Haodi;DAI Shiting;MA Chunmeng;YANG Min;ZHANG Yu(Henan Institutes of Advanced Technology,Zhengzhou University,Zhengzhou,450003,China;State Key Laboratory of Environmental Aquatic Chemistry,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing,100085,China;Department of Municipal and Environmental Engineering,Beijing Jiaotong University,Beijing,100044,China;University of Chinese Academy of Sciences,Beijing,100049,China)

机构地区：[1]郑州大学河南先进技术研究院,郑州450003 [2]中国科学院生态环境研究中心环境水质学国家重点实验室,北京100085 [3]北京交通大学市政与环境工程系,北京100044 [4]中国科学院大学,北京100049

出　　处：《环境化学》2024年第5期1445-1454,共10页Environmental Chemistry

基　　金：国家自然科学基金(22076203)资助.

摘　　要：畜禽粪便源有机肥是农田土壤中抗生素母体及转化产物污染的重要来源之一.为研究畜禽粪便源有机肥中四环素类抗生素及转化产物的残留情况,在全国8个省(市)采集55个有机肥样品,采用超高效液相色谱-串联质谱法(UPLC-MS/MS),分析畜禽粪便源有机肥中四环素、强力霉素、金霉素和土霉素4种四环素类抗生素及10种转化产物的分布特征.结果表明,四环素、强力霉素、金霉素和土霉素在55个有机肥样品中均有检出,浓度分别为1.63-4348μg·kg^(-1)、ND-11451μg·kg^(-1)、ND-30300μg·kg^(-1)、7.11-55008μg·kg^(-1).10种转化产物中,差向异构体是主要的转化产物.不同畜禽粪便来源的有机肥中四环素类抗生素及转化产物的浓度存在显著差异,猪粪肥样品中四环素类抗生素及转化产物的浓度高于鸡粪肥、牛粪肥和羊粪肥.畜禽粪便资源化利用模式可能也会影响有机肥中四环素类抗生素的浓度.种养结合模式生产的有机肥中四环素类抗生素及转化产物的浓度显著高于集中处理模式生产的有机肥.进一步研究了典型地区畜禽粪便源有机肥和施肥土壤中的四环素类抗生素及转化产物残留的污染特征,发现四环素类抗生素及转化产物通过施用有机肥进入土壤环境中,差向异构体在施肥土壤中仍是主要的转化产物.畜禽粪便源有机肥和施肥土壤中四环素类抗生素及转化产物的残留不容忽视.Manure-based organic fertilizers are important sources of pollution of antibiotic and transformation products in farmland soil.To study the residues of tetracyclines and transformation products in manure-based organic fertilizers,55 organic fertilizer samples were collected from eight provinces in China.Ultra-performance liquid chromatography-tandem mass spectrometry(UPLCMS/MS)was used to analyze the distribution characteristics of tetracycline,doxycycline,chlortetracycline,oxytetracycline and their transformation products in organic fertilizers.The results showed that tetracycline,doxycycline,chlortetracycline and oxytetracycline were detected in 55 organic fertilizer samples,and the concentrations were 1.63-4348μg·kg^(−1),ND-11451μg·kg^(−1),ND-30300μg·kg^(−1),7.11-55008μg·kg^(−1),respectively.Among the 10 transformation products,the 4-epimers were the main transformation products.There were significant differences in the contents of tetracyclines and their transformation products in organic fertilizers produced from manure of different animal species.The concentrations and detection rates of tetracyclines and their transformation products in swine manure fertilizers were higher than those in chicken manure fertilizers,cattle manure fertilizers and sheep manure fertilizers.The treatment model of resource utilization of livestock manure might also affect the contents of tetracyclines in organic fertilizers.The concentrations of tetracyclines and their transformation products in organic fertilizers produced by the model of combination of farming and animal husbandry were significantly higher than those produced by the mode of centralized treatment.The pollution characteristics of tetracyclines and their transformation products in manure-based organic fertilizers and fertilized soil in typical areas were further studied.The results showed that the 4-epimers were still the main transformation products in the fertilized soil.Therefore,the residues of tetracyclines and their transformation products in

关 键 词：畜禽粪便源有机肥 施肥土壤 四环素类抗生素 转化产物 

分 类 号：X-1[环境科学与工程] O6[理学—化学]