顶空气相色谱法测定硫化氢中毒血中的甲硫醚和甲硫醇  

Determination of dimethyl sulfide and methyl mercaptan in blood by headspace gas chromatography

作　　者：秦歌 吴小军 王继芬[1] 张震 刘天宇[1] 张云峰[2] QIN Ge;WU Xiaojun;WANG Jifen;ZHANG Zhen;LIU Tianyu;ZHANG Yunfeng(School of Investigation,People’s Public Security University of China,Beijing,100038,China;Institute of Forensic Science,Ministry of Public Security,Beijing,100038,China)

机构地区：[1]中国人民公安大学侦查学院,北京100038 [2]公安部物证鉴定中心,北京100038

出　　处：《环境化学》2024年第5期1565-1572,共8页Environmental Chemistry

基　　金：中国人民公安大学拔尖创新人才培养经费支持研究生科研创新一般项目(2023yjsky009);中央级基本科研业务费(2020JB011)资助.

摘　　要：甲硫醚和甲硫醇作为硫化氢进入人体之后的主要代谢产物,具有一定的标识作用.针对血液中甲硫醚和甲硫醇的检验进行研究,建立了顶空气相色谱检验方法.实验采用对含硫化合物具有高灵敏度的火焰光度检测器(FPD),同时发现向0.5 mL血样中加入0.15 g氯化钠可使检出效率得到提升.本方法血中甲硫醚的标准曲线范围为0.01—2.00μg·mL^(−1),相关系数(R2)为0.997,检出限为0.003μg·mL^(−1),定量限为0.01μg·mL^(−1).虽然甲硫醇由于自身沸点太低,不适合对其进行定量分析,但对其进行定性检测,也可为硫化氢中毒提供一定的依据.本研究建立的方法可直接应用于血液中微量甲硫醚和甲硫醇的检测,从而为硫化氢中毒案件的检验鉴定提供依据.Methyl sulfide and methyl mercaptan,as the main metabolites of hydrogen sulfide after entering the human body,have certain marking effects.A headspace gas chromatographic method was developed for the detection of methyl sulfide and methyl mercaptan in blood.A flame photometric detector(FPD)with high sensitivity for sulfur-containing compounds was used,and it was found that the addition of 0.15 g sodium chloride to 0.5 mL of blood sample resulted in improved detection efficiency.The standard curve range of this method was 0.01—2.00μg·mL^(−1),the correlation coefficient(R2)was 0.997,the limit of detection was 0.003μg·mL^(−1),and the limit of quantification was 0.01μg·mL^(−1).Although methyl mercaptan is not suitable for quantitative analysis due to its low boiling point,its qualitative detection can also provide a basis for hydrogen sulfide poisoning.The method developed in this study can be directly applied to the determination of trace amounts of methyl mercaptan and methyl mercaptan in blood,thus providing a basis for the identification of hydrogen sulfide poisoning cases.

关 键 词：顶空气相色谱法 甲硫醚 甲硫醇 硫化氢 

分 类 号：X-1[环境科学与工程] O6[理学—化学]