基于基因表达芯片和实验验证葛根素降低膀胱尿路上皮癌T24细胞IL6表达及抑制细胞增殖的作用  

Puerarin decreases IL6 expression and inhibits cell proliferation in bladder urothelial carcinoma T24 cells

作　　者：马雨阳 许浩 潘登 王海跞 徐鹏[3] 王靖凯 庞昆 韩从辉[1,2] MA Yu-yang;XU Hao;PAN Deng;WANG Hai-luo;XU Peng;WANG Jing-kai;PANG Kun;HAN Cong-hui(Graduate School,Bengbu Medical College,Bengbu Anhui 233030;Xuzhou Center Hospital,Xuzhou Jiangsu 221009;Graduate School,Jiangsu University,Zhenjiang Jiangsu 212013)

机构地区：[1]蚌埠医学院研究生院,安徽蚌埠233030 [2]徐州市中心医院,江苏徐州221009 [3]江苏大学研究生院,江苏镇江212013

出　　处：《中南药学》2024年第5期1186-1193,共8页Central South Pharmacy

基　　金：国家自然科学基金项目(No.82004110);中国博士后科学基金面上项目(No.2022M722674);徐州市医学后备人才项目(No.XWRCHT20220009);蚌埠医学院2022年度研究生科研创新计划150项资助立项项目(No.Byycx22129)。

摘　　要：目的探讨葛根素抑制膀胱尿路上皮癌T24细胞增殖的作用机制及潜在作用靶点。方法CCK8实验验证葛根素对T24细胞的增殖抑制作用。火山图和热图对芯片数据进行质量控制。对基因表达芯片实验得到的差异表达基因列表进行KEGG、GO通路富集分析、蛋白质相互作用(PPI)和上游转录因子预测,得到关键作用靶点及通路并进行相关验证。结果葛根素抑制T24细胞的增殖活力,这种抑制作用和浓度成正比,半数抑制浓度为218μmol·L^(-1)。KEGG通路富集分析显示差异基因主要富集在细胞生长和增殖相关通路,GO通路富集分析显示差异基因主要富集在蛋白质折叠、DNA复制和肿瘤细胞坏死相关通路。PPI分析得到关键作用靶点IL6。IL6在病理分期Ⅲ、Ⅳ期的表达明显高于Ⅱ期,并且PCR实验证明葛根素可降低T24细胞中IL6的表达。GSEA分析显示IL6与上皮细胞增殖相关。上游转录因子预测得到CENPA,分子对接结果显示葛根素和CENPA之间对接良好。结论葛根素能抑制T24细胞的增殖活力,其作用机制可能是葛根素和CENPA结合,降低IL6的表达进而影响蛋白质折叠、DNA复制、肿瘤细胞坏死和增殖相关通路,最终抑制T24细胞增殖。Objective To determine the mechanism and potential targets of puerarin,when inhibiting the proliferation of bladder urothelial carcinoma T24 cells.Methods The inhibitory effect of puerarin on the proliferation of T24 cells was demonstrated by CCK8 assay.The differentially expressed gene list(DEGL)was obtained by gene expression microarray.Volcano maps and heat maps were used for the quality control of chip data.KEGG,GO pathway enrichment analysis,protein-protein interaction(PPI)and upstream transcription factor prediction were performed to obtain key targets and pathways,and then further validated them.Results Puerarin inhibited the proliferation of T24 cells,whose inhibitory effect was proportional to its concentration,and the half inhibitory-concentration was 218μmol·L^(-1).KEGG pathway enrichment analysis showed that DEGL was mainly enriched in cell growth and proliferation-related pathways,and GO showed that DEGL was enriched in protein folding,DNA replication and tumor cell necrosis-related pathways.PPI yielded the key target IL6.The expression of IL6 in stageⅢandⅣwas obviously higher than that in stageⅡ,and PCR experiments showed that puerarin reduced the expression of IL6 in T24 cells.GSEA analysis showed that IL6 was associated with epithelial cell proliferation.CENPA was predicted by upstream transcription factors,and the molecular docking showed good docking between puerarin and CENPA.Conclusion Puerarin can inhibit the proliferation of T24 cells,whose mechanism may be via the binding of puerarin and CENPA to reduce the expression of IL6 and affect protein folding,DNA replication,tumor cell necrosis and proliferation-related pathways,and ultimately inhibit T24 cell proliferation.

关 键 词：膀胱尿路上皮癌 葛根素 IL6 T24细胞 细胞增殖 

分 类 号：R285[医药卫生—中药学]