藏药五味清热汤散的HPLC指纹图谱及5种成分含量测定方法的研究  

HPLC fingerprint and content determination of 5 components in Tibetan medicine Wuwei Qingre decoction powder

作　　者：姚新民 谢和兵 朱业锦 尼玛次仁 白玛旦增 YAO Xin-min;XIE He-bing;ZHU Ye-jin;Ni-ma-ci-ren;Bai-ma-dan-zeng(School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230013;Tibet Shenhou Pharmaceutical Co.,Ltd,Xigaze Tibet 857000;Jiangsu Shenhou Pharmaceutical Research Co.,Ltd,Nantong Jiangsu 226133;Yangtze Delta Drug Advanced Research Institute,Nantong Jiangsu 226133;School of Medicine,Nanjing University of Traditional Chinese Medicine,Nanjing 210023)

机构地区：[1]安徽中医药大学药学院,合肥230013 [2]西藏神猴药业有限责任公司,西藏日喀则857000 [3]江苏神猴医药研究有限公司,江苏南通226133 [4]海门长三角药物高等研究院,江苏南通226133 [5]南京中医药大学医学院,南京210023

出　　处：《中南药学》2024年第5期1323-1328,共6页Central South Pharmacy

基　　金：西藏自治区科学技术厅-日喀则市人民政府区域科技协同创新专项项目(No.QYXTZX-RKZ2022-07)。

摘　　要：目的 建立藏药五味清热汤散的HPLC指纹图谱并测定指标成分没食子酸、没食子酸甲酯、诃子联苯酸、诃子酸、鞣花酸的含量。方法 采用Ultimate XB-C18液相色谱柱(4.6 mm×250 mm,5 μm),以0.1%磷酸水溶液(A)-甲醇(B)为流动相,梯度洗脱,流速1 mL·min^(-1),检测波长270 nm,柱温30℃,进样量10 μL。结果 五味清热汤散指纹图谱中标识出18个共有峰,指纹图谱相似度>0.99;指标成分没食子酸、没食子酸甲酯、诃子联苯酸、诃子酸和鞣花酸线性范围分别为2.76~55.17 μg·mL^(-1)(r=0.9998)、1.56~31.13 μg·mL^(-1)(r=0.9997)、5.51~110.17 μg·mL^(-1)(r=0.9999)、7.27~145.31 μg·mL^(-1)(r=0.9998)、0.90~18.08 μg·mL^(-1)(r=0.9997);精密度、重复性、稳定性试验的RSD均小于2.0%(n=6);平均加样回收率分别为98.27%(RSD=0.72%)、98.31%(RSD=0.94%)、96.48%(RSD=0.73%)、96.86%(RSD=0.80%)、96.07%(RSD=0.81%)。8批样品中没食子酸、没食子酸甲酯、诃子联苯酸、诃子酸和鞣花酸的含量分别为7.85~8.25、4.02~4.23、16.85~17.77、23.55~24.51、3.09~3.18 mg·g^(-1)。结论 建立的指纹图谱和指标成分含量测定方法具有简便、准确、稳定、快速等优点,可为五味清热汤散的质量评价提供参考。Objective To establish the HPLC fingerprint of the Tibetan medicine Wuwei Qingre decoction powder and determine the content of gallic acid,methyl gallate,chebulate tannic acid,chebulate acid and ellagic acid.Methods An Ultimate XB-C18 liquid chromatographic column(4.6 mm×250 mm,5μm)was used,with 0.1%phosphoric acid aqueous solution(A)-methanol(B)as the mobile phase,gradient elution,flow rate of 1 mL·min^(-1),detection wavelength of 270 nm,column temperature of 30℃,and injection volume of 10μL.Results Totally 18 common peaks in the fingerprint of Wuwei Qingre decoction powder were confirmed,and the similarity of fingerprint was higher than 0.99,The linear ranges of gallic acid,methyl gallate,chebulate tannic acid,chebulate acid,and ellagic acid were 2.76~55.17μg·mL^(-1)(r=0.9998),1.56~31.13μg·mL^(-1)(r=0.9997),5.51~110.17μg·mL^(-1)(r=0.9999),7.27~145.31μg·mL^(-1)(r=0.9998),and 0.90~18.08μg·mL^(-1)(r=0.9997),respectively.The RSDs of precision,repeatability and stability tests were all less than 2.0%(n=6).The average recoveries were 98.27%(RSD=0.72%),98.31%(RSD=0.94%),96.48%(RSD=0.73%),96.86%(RSD=0.80%)and 96.07%(RSD=0.81%)respectively.The content of the 5 components in 8 batches sample was 7.85~8.25,4.02~4.23,16.85~17.77,23.55~24.51,and 3.09~3.18 mg·g-1,respectively.Conclusion The fingerprint and content determination method is simple,accurate,stable and rapid,which can provide reference for the quality control of Wuwei Qingre decoction powder.

关 键 词：五味清热汤散 诃子酸 鞣花酸 高效液相色谱法 指纹图谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]