基于HPLC和化学计量法分析蜘蛛香不同部位3类化合物10种成分差异研究  

Differences of 10 components in 3 types of compounds from different parts of Valeriana jatamansi Jones based on HPLC and chemometrics

作　　者：程盛勇 胡光线 付志丽 沈祥春 杨军[4] 罗喜荣 CHENG Sheng-yong;HU Guang-xian;FU Zhi-li;SHEN Xiang-chun;YANG Jun;LUO Xi-rong(Department of Medicine Basic Sciences,Guizhou Nursing Vocational College,Guiyang 550025;Guizhou Zhongguan Biotechnology Co.,Ltd.,Guiyang 550081;School of Pharmaceutical Sciences,Guizhou Medical University,Guiyang 561113;Institute of Geochemistry,Chinese Academy of Sciences,Guiyang 550081)

机构地区：[1]贵州护理职业技术学院基础医学部,贵阳550025 [2]贵州中观生物技术有限公司,贵阳550081 [3]贵州医科大学药学院,贵阳561113 [4]中国科学院地球化学研究所,贵阳550081

出　　处：《中南药学》2024年第5期1339-1344,共6页Central South Pharmacy

基　　金：贵州省科技创新基地(黔科合中引地[2023]003);贵州省科技计划项目(黔科合基础-ZK[2023]一般126);贵州护理职业技术学院硕士基金课题(No.gzhlyj2023-16);贵州护理职业技术学院硕士基金课题(No.gzhlyj2022-06)。

摘　　要：目的 对蜘蛛香不同部位中酚酸类(新绿原酸、绿原酸、咖啡酸、异绿原酸B、异绿原酸A、异绿原酸C),黄酮类(橙皮苷、蒙花苷)及缬草三酯类(乙酰缬草三酯、缬草三酯)化合物含量进行测定,结合化学模式识别比较含量差异。方法 采用HPLC法对蜘蛛香地上部位(叶)和地下部位(根茎和根)中10种成分含量进行测定,对结果进行聚类分析、主成分分析和正交偏最小二乘判别分析。结果 地上部位10种成分平均含量绿原酸>异绿原酸A>蒙花苷>缬草三酯>橙皮苷>异绿原酸C>乙酰缬草三酯>异绿原酸B>新绿原酸>咖啡酸,地下部位10种成分平均含量缬草三酯>异绿原酸A>橙皮苷>绿原酸>乙酰缬草三酯>异绿原酸C>异绿原酸B>蒙花苷>新绿原酸>咖啡酸;蜘蛛香不同部位酚酸类和黄酮类含量均为地上部位>地下部位,缬草三酯类含量为地下部位>地上部位;造成蜘蛛香不同部位差异的标志物为缬草三酯、蒙花苷、橙皮苷。结论 蜘蛛香各部位样品中10个成分的含量存在较大差异;蜘蛛香的非药用部位也含有大量的酚酸类、黄酮类和缬草三酯类,应充分利用,避免中药资源的浪费。Objective To determine the content of 10 components in 3 types of compounds,including phenolic acids(neochlorogenic acid,chlorogenic acid,caffeic acid,isochlorogenic acid B,isochlorogenic acid A,and isochlorogenic acid C),flavonoids(hesperidin and linarin),and valerian triglycerides(valepotriate and acevaltrate)from different parts of Valeriana jatamansi,and compare the content differences by combining chemical pattern recognition.Methods The content of 10 components in the aboveground parts(leaves)and underground parts(rhizomes and roots)of Valeriana jatamansi was detected by HPLC.Hierarchical clustering analysis,principal component analysis and orthogonal partial least squares discriminant analysis were conducted.Results The average content order of 10 components in the aboveground part were as follows:chlorogenic acid>isochlorogenic acid A>linarin>valepotriate>hesperidin>isochlorogenic acid C>acevaltrate>isochlorogenic acid B>neochlorogenic acid>caffeic acid,while the average content of 10 components in the underground part were valepotriate>isochlorogenic acid A>hesperidin>chlorogenic acid>acevaltrate>isochlorogenic acid C>isochlorogenic acid B>linarin>neochlorogenic acid>caffeic acid.The content of both phenolic acids and flavonoids in the different parts of Valeriana jatamansi were aboveground part>underground part,and the content of valerian triglycerides was underground part>aboveground parts.The markers that caused differences included valepotriate,linarin,and hesperidin.Conclusion There are obvious differences in the content of 10 components in different parts of Valeriana jatamansi.The non-medicinal parts of Valeriana jatamansi also contain a large amount of phenolic acids,flavonoids,and valepotriates,which may be utilized.

关 键 词：蜘蛛香 不同部位 HPLC 化学计量学 含量测定 

分 类 号：R284.1[医药卫生—中药学]