硼硅酸盐生物活性玻璃的矿化及机理初探  

Preliminary investigation on the mineralization mechanism of borosilicate bioactive glass

作　　者：周杰[1] 王会[1] 崔旭[1] 黄文旵[1] 王德平[1] 周萘[1] Zhou Jie;Wang Hui;Cui Xu;Huang Wenhai;Wang Deping;Zhou Nai(School of Materials Science and Engineering,Tongji University,Shanghai,201804,China)

机构地区：[1]同济大学材料科学与工程学院,上海201804

出　　处：《生物骨科材料与临床研究》2024年第3期1-7,共7页Orthopaedic Biomechanics Materials and Clinical Study

摘　　要：目的探讨硼硅酸盐生物活性玻璃体外矿化成类骨层状结构的机理。方法模拟体内环境,将硅酸盐玻璃(0B)、硼硅酸盐玻璃(2B)和硼酸盐玻璃(3B)浸泡于SBF溶液和含有明胶的SBF溶液中1周;随后采用质量损失分析、SEM、EDS、XRD及FTIR等测试方法对多种生物活性玻璃的矿化产物进行系统分析与比较;采用固体核磁NMR对生物玻璃进行玻璃结构分析。结果浸泡1周后,SEM观察到硼硅酸盐玻璃(2B)和不含硅的硼酸盐玻璃(3B)表面产生层状矿化产物,而硅酸盐玻璃(0B)表面只有一层矿化产物层;XRD和FTIR结果显示3种玻璃的表面产物均为含有部分羟基磷灰石的钙磷化合物;固体核磁波谱(11B)测试结果表明硼硅酸盐玻璃(2B)和不含硅的硼酸盐玻璃(3B)都存在大量[BO_(3)]平面三角体。最后,当硼硅酸盐玻璃(2B)浸泡在含有明胶的SBF溶液后,其矿化产物仍能维持层状结构;通过EDS分析可知形成明胶有机层和含羟基磷灰石的无机层相互堆积的特殊层状结构。结论2B和3B玻璃内部存在大量[BO_(3)]平面三角体所构造的二维平面结构。矿化过程中,不溶性反应物沉积在二维平面结构上,从而使矿化产物呈现层状结构。在浸泡液中添加一定量的明胶后,其可以有效地与硼硅酸盐玻璃的层状矿化结构相互匹配结合,最终堆叠形成有机相层与无机层相互交错的特殊结构。Objective To investigate the mineralization mechanism for the formation of a bone-like layered structure for borosilicate bioactive glass in vitro.Methods To simulate the internal environment,silicate glass(0B),borosilicate glass(2B)and borate glass(3B)were immersed in SBF solution or SBF solution containing gelatin for one week.The morphologies and phases of the mineralized products formed by the bioactive glass in SBF were systematically characterized and analyzed by mass loss analysis,SEM,EDS,XRD and FTIR.The glass structure of 2B and 3B glass was analyzed by solid state nuclear magnetic resonance.Results After soaking a week,the SEM results showed that the surfaces of borosilicate glass(2B)and silica-free borate glass(3B)were covered with a layered mineralisation coating,whereas the surface of silicate glass was not.XRD and FTIR results indicated that all of the three groups glasses contained the partial hydroxyapatite compounds of calcium phosphate.Solid-state nuclear magnetic testing resonance showed that a large number of[BO_(3)]planar triangles were present in groups 2B and 3B.After immersion in the gelatin-containing SBF solution,the borosilicate glass(2B)still maintained the layered structure,and EDS analysis showed that the special layered structure was the mutually accumulated by the organic layers of gelatin and inorganic layers containing hydroxyapatite.Conclusion It was found that the two-dimensional met structure constructed by a lot of[BO_(3)]plane triangles existed in 2B and 3B glasses.During the mineralization process,insoluble reactants were deposited on a two-dimensional plane structure,resulting in a layered structure of the mineralization products.The gelatin added to SBF can effectively adapt and combine with the layered mineralized structure of borosilicate glass,ultimately stacking a special intersected structure of organic and inorganic layers.

关 键 词：硼硅酸盐生物活性玻璃 骨修复材料 矿化产物 层状结构 

分 类 号：R318.08[医药卫生—生物医学工程]