内侧前额叶皮质神经元参与二苯基环丙烯酮诱导的小鼠瘙痒  

Medial prefrontal cortex neurons are involved in diphenylcycloacrylonone induced itch in mice

作　　者：刘静雯 朱媛媛 戴梓怡 黄静 白占涛 LIU Jingwen;ZHU Yuanyuan;DAI Ziyi;HUANG Jing;BAI Zhantao(School of Life Sciences and Peptide Resource Medicine Research Center,Shaanxi Provincial Engineering Research Center for Conservation and Utilization of Regional Biological Resources,Yan’an University,Yan’an 716000;Department of Neurobiology,School of Basic Medicine,Air Force Medical University,Xi'an 710032,China;The 5th Battalion,School of Basic Medical Sciences,Air Force Medical University,Xi’an 710032,China)

机构地区：[1]延安大学生命科学学院多肽资源药物研究中心,陕西省区域生物资源保育与利用工程技术研究中心,延安716000 [2]空军军医大学基础医学院神经生物学教研室,西安710032 [3]空军军医大学基础医学院学员五大队,西安710032

出　　处：《神经解剖学杂志》2024年第2期137-144,共8页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(82071235);陕西省杰青基金(2021JC-32)

摘　　要：目的:建立接触性皮炎瘙痒模型,检测痒行为和焦虑、抑郁样行为;观察内侧前额叶皮质(mPFC)神经元激活情况,为其参与痒觉信息的调控提供形态学证据。方法:采用二苯基环丙烯酮(DCP)涂抹小鼠颈背部构建接触性皮炎瘙痒模型,检测小鼠的搔抓行为。开展旷场及悬尾行为学实验,探究小鼠在接触性皮炎状态下的焦虑、抑郁样行为。运用免疫荧光染色技术探究接触性皮炎模型小鼠mPFC内c-Fos及p-ERK1/2阳性细胞分布情况。结果:成功建立接触性皮炎瘙痒模型,模型组小鼠半小时内的抓挠次数明显高于对照组(P<0.05)。旷场结果显示接触性皮炎模型组小鼠在中央区域的时间和距离显著减少。悬尾实验结果提示接触性皮炎模型组小鼠不动的时间显著增加(P<0.05)。与对照组相比,接触性皮炎实验组小鼠mPFC内c-Fos阳性细胞和p-ERK1/2阳性细胞数量显著增加,具有统计学差异(P<0.05)。结论:接触性皮炎实验组小鼠抓挠次数显著增多,并且诱发焦虑、抑郁样行为。接触性皮炎状态下,mPFC内神经元激活,为mPFC神经元参与接触性皮炎瘙痒及共患焦虑、抑郁等情感障碍提供了形态学依据。Objective:To establis contact dermatitis pruritus model and detect itch,anxiety and depression-like behaviors.To observe the activation of the medial prefrontal cortex(mPFC)neurons and provide morphological evidence for its involvement in the regulation of itch perception information.Methods:A model of contact dermatitis pruritus was constructed with diphenylcyclopyrenone(DCP)applied on the neck and back of the mouse,and the itch perception video tracking system was used to detect the scratching behavior.Field relief and suspended tail behavioral experiments were conducted to explore the anxiety and depression-like behaviors of mice under contact dermatitis.The distribution of c-Fos and p-ERK1/2 positive cells in the mPFC of contact dermatitis model mice was investigated by immunofluorescence staining.Results:The contact dermatitis pruritus model was successfully established,and the bouts of scratching in the contact dermatitis model group was significantly higher than that in the control group(P<0.05).The results of open field test showed that mice in the contact dermatitis model group had a significant reduction in time and distance in the central area.The results of the tail suspension experiment suggested that the time of immobility increased significantly in the contact dermatitis model group(P<0.05).There were more c-Fos positive and p-ERK1/2 positive cells in the mPFC compared with the control group(P<0.05).Conclusion:The mice in the contact dermatitis group increased scratching times and induced anxiety and depression-like behaviors.In the contact dermatitis state,mPFC neurons activate,providing morphological evidence for the involvement of mPFC neurons in contact dermatitis pruritus and accompanying affective disorders such as anxiety and depression.

关 键 词：接触性皮炎 瘙痒 内侧前额叶皮质 C-FOS P-ERK1/2 小鼠 

分 类 号：R758.22[医药卫生—皮肤病学与性病学]