跑台训练促进可控性皮质损伤大鼠髓鞘再生  

Treadmill exercise promotes myelin regeneration in rats with controlled cortical impact

作　　者：高丹丹 朱昆源 闭士峻 高阳 GAO Dandan;ZHU Kunyuan;BI Shijun;GAO Yang(Department of Neurosurgery,General Hospital of Northern Theater Command of Chinese People’s Liberation Army,Shenyang 110016,China)

机构地区：[1]中国人民解放军北部战区总医院神经外科,沈阳110016

出　　处：《神经解剖学杂志》2024年第2期241-244,256,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(82071481)。

摘　　要：目的:研究跑台训练对可控性皮质损伤(CCI)大鼠髓鞘再生的影响。方法:通过撞击法制备CCI大鼠模型,进行8周跑台训练,采用改良的神经功能严重程度评分法检测神经功能缺损,利用平衡木实验检测大鼠的运动功能,使用酶联免疫吸附试验(ELISA)和RT-qPCR方法检测运动皮质中(BDNF)的表达,利用Western Blot方法检测胼胝体中髓鞘碱性蛋白(MBP)、髓鞘少突胶质细胞糖化蛋白(MOG)和髓鞘蛋白脂蛋白(PLP)的表达。结果:跑台训练改善了CCI损伤大鼠的神经功能评分和运动功能,增加了运动皮质中BDNF的表达,同时MBP、MOG和PLP表达上调。结论:跑台训练通过上调BDNF促进髓鞘修复,从而改善CCI损伤引起的神经功能缺损。Objective:To study the effect of treadmill training on myelin regeneration in rats with controlled cortical impact(CCI).Methods:The rat CCI model of was prepared by impinging method,and the rat model was trained by running on the treadmill for 8 weeks.The neural function deficit was detected by the modified neurological function severity scoring method,the motor function of the rats was detected by the balance beam test,and the expression of BDNF was detected by enzyme-linked immunosorbent assay(ELISA)and RT-qPCR.The expressions of myelin basic protein(MBP),myelin oligodendrocyte glycating protein(MOG)and myelin protein lipoprotein(PLP)in corpus callosum were detected by Western Blot.Results:Treadmill training improved the neural function score and motor function of CCI injured rats,increased the expression of BDNF in cortex,and upregulated the expression of MBP,MOG,and PLP.Conclusion:Treadmill training promotes myelin repair by upregating BDNF,thereby improving the neural function deficit caused by CCI injury.

关 键 词：可控性皮质损伤 跑台训练 髓鞘再生 鸢尾素 少突胶质细胞 大鼠 

分 类 号：R651.2[医药卫生—外科学]