死精子症患者精子存活率与精液细菌培养阳性率的相关性研究  

Correlation between sperm survival rate and positive rate of sperm germiculture in patients with necrozoospermia

作　　者：李建波 闵保华 马旭辉 杨涛 兰辉 王晓红 LI Jianbo;MIN Baohua;MA Xuhui;YANG Tao;LAN Hui;WANG Xiaohong(Center for Reproductive Medicine,Department of Obstetrics and Gynecology,Tangdu Hospital,Air Force Medical University,Xi'an 710038,China)

机构地区：[1]空军军医大学唐都医院妇产科生殖医学中心,陕西西安710038

出　　处：《空军军医大学学报》2024年第6期673-677,共5页Journal of Air Force Medical University

基　　金：国家自然科学基金(82071717)。

摘　　要：目的分析死精子症患者精液细菌培养阳性率与精子存活率之间的相关性。方法回顾性分析2022年3月至9月于空军军医大学唐都医院妇产科生殖医学中心男科就诊,精液常规检查2次以上,通过伊红染色确诊为死精子症的患者110例(死精子症组),按照精子存活率将死精子症患者分为轻度死精子症(35%<存活率≤58%)40例,中度死精子症(15%<存活率≤35%)42例和重度死精子症(0%<存活率≤15%)28例;另外将20例精液检查正常的男性作为对照组。对入组的患者同时行精液常规检查和精液细菌培养,统计细菌培养阳性率的情况。结果经过精液细菌培养,各组的阳性率分别为:轻度死精子症组19例、中度死精子症组26例、重度死精子症组23例、对照组3例;死精子症组患者细菌培养阳性率(68/110)与对照组(3/20)比,组间存在极显著差异(χ^(2)=14.96,P<0.01);重度死精子症组(23/28)的阳性率显著高于轻度死精子症组(19/40)(χ^(2)=8.37,P<0.01);而中度死精子症组(26/42)与重度死精子症组(23/28)和轻度死精子症组(19/40)间均无统计学差异(P>0.05);另外发现,精液细菌培养阳性率随着精子存活率下降而显著上升,尤其是当精子存活率低于15%时,细菌培养阳性率显著增高(χ^(2)=6.57,P<0.05)。结论死精子症患者精液细菌培养阳性率与精子存活率间存在相关性,尤其是精子存活率低于15%时,所以建议将精液细菌培养作为重度死精子症患者的临床常规检查项目。Objective To analyze the correlation between sperm survival rate and positive rate of sperm germiculture in patients with necrozoospermia.Methods A retrospective analysis was performed on 110 patients diagnosed with necrozoospermia(necrozoospermia group)by eosin staining after semen examination for more than 2 times in Center for Reproductive Medicine,Department of Obstetrics and Gynecology,Tangdu Hospital,Air Force Medical University from March to September 2022.According to the sperm survival rate,the patients were divided into 3 groups:40 cases of mild necrozoospermia(35%<survival rate≤58%),42 cases of moderate necrozoospermia(15%<survival rate≤35%)and 28 cases of severe necrozoospermia(0%<survival rate≤15%).In addition,20 men with normal sperm survival rate were used as control group.Semen examination and sperm germiculture were performed on the enrolled patients,and the positive rate of germiculture was counted.Results After sperm germiculture,the positive rates of each group were as follows:19 cases in the mild necrozoospermia group,26 cases in the moderate necrozoospermia group,23 cases in the severe necrozoospermia group and 3 cases in the control group.The positive rate of germiculture in the necrozoospermia group(68/110)was significantly different from that in the control group(3/20)(χ^(2)=14.96,P<0.01).The positive rate of the severe necrozoospermia group(23/28)was significantly higher than that of the mild necrozoospermia group(19/40)(χ^(2)=8.37,P<0.01).However,there was no statistical difference between the moderate necrozoospermia group(26/42)and the severe necrozoospermia group(23/28),as well as the mild necrozoospermia group(19/40)(P>0.05).In addition,the positive rate of sperm germiculture significantly increased with the decrease of sperm survival rate,and especially when the sperm survival rate was less than 15%,the positive rate of germiculture significantly increased(χ^(2)=6.57,P<0.05).Conclusion There is a correlation between the positive rate of sperm germiculture and sperm surviva

关 键 词：死精子症 精道感染 细菌培养 不育症 

分 类 号：R698.2[医药卫生—泌尿科学]