纳米复合材料修饰的电化学免疫传感器检测血清中肺癌标志物CYFRA21-1  

Detection of Lung Cancer Marker CYFRA21-1 in Serum by Electrochemical Immunosensor Modified with Nanocomposite

作　　者：史峰峰 蒋裕婷 龚国傲 覃江阳 梅勇[1] SHI Fengfeng;JIANG Yuting;GONG Guoao;QIN Jiangyang;MEI Yong(Key Laboratory of Occupational Hazards Identification and Control in Hubei Province,School of Public Health,Wuhan University of Science and Technology,Wuhan 430065,China)

机构地区：[1]武汉科技大学公共卫生学院职业危害识别与控制湖北省重点实验室,武汉430065

出　　处：《理化检验（化学分册）》2024年第5期495-501,共7页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：构建基于纳米复合材料修饰的电化学免疫传感器,用以检测肺癌标志物细胞角蛋白19片段抗原21-1(CYFRA21-1)。选用有序介孔碳CMK-3与羧基化多壁碳纳米管(CMWCNTs)结合,提升电子传递速率,实现电化学信号放大,联合金纳米粒子(AuNPs)修饰玻碳电极制得电化学免疫传感器。AuNPs与CMK-3@CMWCNTs均匀复合,并可通过Au-S键与CYFRA21-1抗体结合,为抗体提供大量的生物结合位点,提高了该传感器的灵敏度,进而通过抗原-抗体特异性反应实现对血清中CYFRA21-1的检测。扫描电子显微镜表征显示,AuNPs颗粒嵌入CMK-3@CMWCNTs的表面和空隙中,循环伏安法(CV)曲线的变化趋势表明电化学免疫传感器灵敏有效。采用构建的电化学免疫传感器对血清中CYFRA21-1进行检测,线性范围为0.5~1×10~4 ng·L^(-1),检出限(3S/N)为0.2 ng·L^(-1),血清样品中CYFRA21-1的加标回收率为91.4%~102%,测定值的相对标准偏差(n=6)均小于7.0%。An electrochemical immunosensor modified with nanocomposite was constructed to detect cytokeratin 19 fragment antigen 21-1(CYFRA21-1)which was a lung cancer marker.Ordered mesoporous carbon CMK-3 was combined with carboxylated multi walled carbon nanotubes(CMWCNTs)to improve the electron transfer rate and achieve electrochemical signal amplification.Au nanoparticles(AuNPs)were combined furtherly to prepare an electrochemical immunosensor on a glassy carbon electrode.AuNPs could be uniformly compounded with CMK-3@CMWCNTs and combined with the CYFRA21-1 antibody through the Au-S bond,providing a large number of biological binding sites for the antibody and improving the sensitivity of the sensor,so as to realize the detection of CYFRA21-1 in serum through antigen-antibody specific reaction.Scanning electron microscope characterization showed that AuNPs were embedded in the surface and pores of [e-mail] trends of cyclic voltammetry(CV)curves showed that the constructed electrochemical immunosensor was sensitive and effective.The linear range of the constructed electrochemical immunosensor for detection of CYFRA21-1 in serum was 0.5-1×104 ng·L-1,with detection limit(3S/N)of 0.2 ng·L-1.The spiked recovery of CYFRA21-1 in the serum sample was in the range of 91.4%-102%,with RSDs(n=6)of the determined values less than 7.0%.

关 键 词：电化学免疫传感器 细胞角蛋白19片段抗原21-1(CYFRA21-1) 金纳米粒子 有序介孔碳 

分 类 号：O657.14[理学—分析化学]